Manual of Methods for General Bacteriology |
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Page 48
For each bacterial pellet or other sample to be embedded — not each agar cube
resulting from the sample - allow at least 6 ml of complete mixture . This volume
will take care of mixtures in steps 4 to 6 of the dehydration and embedment ...
For each bacterial pellet or other sample to be embedded — not each agar cube
resulting from the sample - allow at least 6 ml of complete mixture . This volume
will take care of mixtures in steps 4 to 6 of the dehydration and embedment ...
Page 379
5 M HCI , and allow the mixture to stand at room temperature for 15 min . Add 1
ml of 40 % KOH , shake the contents , and then read in a Klett - Summerson
colorimeter using the 54 filter or in any standard spectrophotometer or
colorimeter at ...
5 M HCI , and allow the mixture to stand at room temperature for 15 min . Add 1
ml of 40 % KOH , shake the contents , and then read in a Klett - Summerson
colorimeter using the 54 filter or in any standard spectrophotometer or
colorimeter at ...
Page 390
Appropriate samPrior to termination of growth in the culture ples are taken from
each fraction and placed in vessel , remove a 5 - ml ( or more ) sample and add
scintillation vials ; the radioactivity is deterto 5 ml of a mixture of cold carrier ...
Appropriate samPrior to termination of growth in the culture ples are taken from
each fraction and placed in vessel , remove a 5 - ml ( or more ) sample and add
scintillation vials ; the radioactivity is deterto 5 ml of a mixture of cold carrier ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York