Manual of Methods for General Bacteriology |
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Page 229
... mutant cells to grow , the addition of penicillin selectively kills the non- mutant cells by interfering with the cell wall synthesis of the actively dividing cells . Under optimal conditions a 1,000 - fold enrichment of mutants to ...
... mutant cells to grow , the addition of penicillin selectively kills the non- mutant cells by interfering with the cell wall synthesis of the actively dividing cells . Under optimal conditions a 1,000 - fold enrichment of mutants to ...
Page 232
... mutants , mutants involving carbohydrate fermentation , morphological var- iants of various types , and other conditional lethal mutants . The most commonly employed indirect tech- niques for detecting such mutants are random colony ...
... mutants , mutants involving carbohydrate fermentation , morphological var- iants of various types , and other conditional lethal mutants . The most commonly employed indirect tech- niques for detecting such mutants are random colony ...
Page 234
... Mutants which respond to none of the pools may be ( i ) mutants requiring some metabolite ( e.g. , a dif- ferent vitamin ) not tested or ( ii ) double mutants requiring a combination of two metabolites not represented in these pools ...
... Mutants which respond to none of the pools may be ( i ) mutants requiring some metabolite ( e.g. , a dif- ferent vitamin ) not tested or ( ii ) double mutants requiring a combination of two metabolites not represented in these pools ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract