Manual of Methods for General Bacteriology |
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Page 270
... obtained by this method should be further purified for restriction endonuclease analysis ( see 15.3.2 ) by using the following ad- ditional steps : 11. Resuspend the pellet obtained in step 9 in 100 μl of 0.010 M Tris at pH 8.0 . Add ...
... obtained by this method should be further purified for restriction endonuclease analysis ( see 15.3.2 ) by using the following ad- ditional steps : 11. Resuspend the pellet obtained in step 9 in 100 μl of 0.010 M Tris at pH 8.0 . Add ...
Page 395
... obtained by difference . Tubes with nominal 50 - ml capacity are best for pellets of about 10 g ( wet weight ) and 15 - ml tubes are best for pellets of about 3 g . Weighing to within 0.01 g is sufficiently accurate for routine assays ...
... obtained by difference . Tubes with nominal 50 - ml capacity are best for pellets of about 10 g ( wet weight ) and 15 - ml tubes are best for pellets of about 3 g . Weighing to within 0.01 g is sufficiently accurate for routine assays ...
Page 505
... obtained by weigh- ing a sample in a tared pan after separation and washing of the cells by filtration or centrifuga- tion . In either case , however , diluent is trapped in the interstitial ( intercellular ) space and con- tributes to ...
... obtained by weigh- ing a sample in a tared pan after separation and washing of the cells by filtration or centrifuga- tion . In either case , however , diluent is trapped in the interstitial ( intercellular ) space and con- tributes to ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract