Manual of Methods for General Bacteriology |
From inside the book
Results 1-3 of 41
Page 41
... pellet twice in Kellenberger Veronal - acetate buffer , centrifuging between washes . 4. Suspend the washed pellet in 1 % osmium tetroxide for 6 to 16 h at room temperature . ( The glutaraldehyde - fixed pellet may be en- robed in agar ...
... pellet twice in Kellenberger Veronal - acetate buffer , centrifuging between washes . 4. Suspend the washed pellet in 1 % osmium tetroxide for 6 to 16 h at room temperature . ( The glutaraldehyde - fixed pellet may be en- robed in agar ...
Page 395
... pellet . do not affect the pellet weight . The final centrif- ugation before solute addition is generally done with a tared centrifuge tube so that the pellet weight can be obtained by difference . Tubes with nominal 50 - ml capacity ...
... pellet . do not affect the pellet weight . The final centrif- ugation before solute addition is generally done with a tared centrifuge tube so that the pellet weight can be obtained by difference . Tubes with nominal 50 - ml capacity ...
Page 396
... pellets and incubation times . 5. Recentrifugation . After each mixture of pellet and test solution has been incubated , it should be centrifuged sufficiently to obtain a cell - free supernatant fluid for assay . The supernatant fluid ...
... pellets and incubation times . 5. Recentrifugation . After each mixture of pellet and test solution has been incubated , it should be centrifuged sufficiently to obtain a cell - free supernatant fluid for assay . The supernatant fluid ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
14 other sections not shown
Other editions - View all
Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract