Manual of Methods for General Bacteriology |
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Page 41
Wash the pellet twice in Kellenberger Veronal - acetate buffer , centrifuging
between washes . 4 . Suspend the washed pellet in 1 % osmium tetroxide for 6 to
16 h at room temperature . ( The glutaraldehyde - fixed pellet may be en - robed
in ...
Wash the pellet twice in Kellenberger Veronal - acetate buffer , centrifuging
between washes . 4 . Suspend the washed pellet in 1 % osmium tetroxide for 6 to
16 h at room temperature . ( The glutaraldehyde - fixed pellet may be en - robed
in ...
Page 395
ASSAY PROCEDURE 395 Pellet weightPellet volumes can be estimated readily
by use of a standard pycnometer or a volumetric flask . For example , suppose
one has a cell pellet that weighs about 3 g and wishes to know its volume .
ASSAY PROCEDURE 395 Pellet weightPellet volumes can be estimated readily
by use of a standard pycnometer or a volumetric flask . For example , suppose
one has a cell pellet that weighs about 3 g and wishes to know its volume .
Page 396
After mixing of pellet and solution , the dextran preparation are sufficiently large
to be suspension should be incubated for ... Of course , it would be posperiod by
use of multiple pellets and incubation sible to label larger dextran molecules with
...
After mixing of pellet and solution , the dextran preparation are sufficiently large
to be suspension should be incubated for ... Of course , it would be posperiod by
use of multiple pellets and incubation sible to label larger dextran molecules with
...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |