Manual of Methods for General Bacteriology |
From inside the book
Results 1-3 of 34
Page 27
... phenol dissolved in the water . Mix , and let stand for several days . Filter before use . Decolorizing solvent : Ethanol , 95 % ( vol / vol ) , 97 ml Hydrochloric acid ( concentrated ) , 3 ml Counterstain : Methylene blue chloride ...
... phenol dissolved in the water . Mix , and let stand for several days . Filter before use . Decolorizing solvent : Ethanol , 95 % ( vol / vol ) , 97 ml Hydrochloric acid ( concentrated ) , 3 ml Counterstain : Methylene blue chloride ...
Page 342
... phenol : Place equal volumes of redis- tilled liquid phenol and water in a well - capped bot- tle , shake well , and remove the phenol layer . Store in a brown bottle at 4 ° C . Prepare fresh for each use from redistilled phenol stored ...
... phenol : Place equal volumes of redis- tilled liquid phenol and water in a well - capped bot- tle , shake well , and remove the phenol layer . Store in a brown bottle at 4 ° C . Prepare fresh for each use from redistilled phenol stored ...
Page 455
... Phenol - cresol mixture : 550 ml of liquefied phenol , 70 ml of m - cresol , and 0.5 g of 8- hydroxyquinoline . Diethyl pyrocarbonate ( this reagent is very un- stable in water ; add it just prior to lysing the cells ) . SSC ( see 22.2 ...
... Phenol - cresol mixture : 550 ml of liquefied phenol , 70 ml of m - cresol , and 0.5 g of 8- hydroxyquinoline . Diethyl pyrocarbonate ( this reagent is very un- stable in water ; add it just prior to lysing the cells ) . SSC ( see 22.2 ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
14 other sections not shown
Other editions - View all
Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract