Manual of Methods for General Bacteriology |
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Page 230
In the first example given below , the use of a simple gradient plate technique is
described which can be modified for the detection of ... Preparation of gradient
plate for isolation Preparation of gradient plates . of antibiotic - resistant mutants .
In the first example given below , the use of a simple gradient plate technique is
described which can be modified for the detection of ... Preparation of gradient
plate for isolation Preparation of gradient plates . of antibiotic - resistant mutants .
Page 236
17 ) at 55°C mix by rotating the tube rapidly between the palms , and pour over
the surface of a fresh LC agar plate ( 13 . 9 . 18 ) . 4 . Incubate the plates
overnight at 37°C and count plaques the next day . 5 . Concentrate the phage
from the ...
17 ) at 55°C mix by rotating the tube rapidly between the palms , and pour over
the surface of a fresh LC agar plate ( 13 . 9 . 18 ) . 4 . Incubate the plates
overnight at 37°C and count plaques the next day . 5 . Concentrate the phage
from the ...
Page 428
the master plate . Nine submaster plates can be prepared from a single master
plate . After growth occurs on the submaster plates , use each plate to print test
plates for nine different carbon sources , followed by a terminal yeast extract agar
...
the master plate . Nine submaster plates can be prepared from a single master
plate . After growth occurs on the submaster plates , use each plate to print test
plates for nine different carbon sources , followed by a terminal yeast extract agar
...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York