Manual of Methods for General Bacteriology |
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Page 238
... prepared with sterile water and stored at -20 ° C . The stock salt solutions ( 0.4 M MgCl2-1.65 M KCl ) and phos- phate buffer ( 0.2 M , pH 7.4 ) can likewise be prepared , autoclaved , and stored in a refrigera- tor . The S - 9 mix is ...
... prepared with sterile water and stored at -20 ° C . The stock salt solutions ( 0.4 M MgCl2-1.65 M KCl ) and phos- phate buffer ( 0.2 M , pH 7.4 ) can likewise be prepared , autoclaved , and stored in a refrigera- tor . The S - 9 mix is ...
Page 264
... prepared freshly ( at least every week ) , since cysteine is rapidly oxidized to cystine ( which is very insoluble ) . Alternatively , the stock solution of cysteine can be overlaid with sterile mineral oil after autoclaving . f Prepared ...
... prepared freshly ( at least every week ) , since cysteine is rapidly oxidized to cystine ( which is very insoluble ) . Alternatively , the stock solution of cysteine can be overlaid with sterile mineral oil after autoclaving . f Prepared ...
Page 334
... prepared without glucose ( 16.1.1 ) . De- termine the concentration of glucose in the sam- ples from a standard curve prepared by plotting the absorbances of the standards versus the concentration of glucose . 17.2.3 . D - Glucose A ...
... prepared without glucose ( 16.1.1 ) . De- termine the concentration of glucose in the sam- ples from a standard curve prepared by plotting the absorbances of the standards versus the concentration of glucose . 17.2.3 . D - Glucose A ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract