Manual of Methods for General Bacteriology |
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Page 55
... protoplast formation . Phase micros- copy is used to monitor protoplast formation . Low levels of Mg2 + or Ca2 + ( in the range of 0.5 to 5 mM ) may be required to stabilize the pro- toplasts . Lysozyme digestion can be carried out ...
... protoplast formation . Phase micros- copy is used to monitor protoplast formation . Low levels of Mg2 + or Ca2 + ( in the range of 0.5 to 5 mM ) may be required to stabilize the pro- toplasts . Lysozyme digestion can be carried out ...
Page 402
... protoplast volume . Generally , estimates from the two sources agree reasonably well . The elasticity of the cell wall does not affect the swelling of isolated protoplasts , which have been used often for transport studies , and is ...
... protoplast volume . Generally , estimates from the two sources agree reasonably well . The elasticity of the cell wall does not affect the swelling of isolated protoplasts , which have been used often for transport studies , and is ...
Page 403
... protoplasts can also be stabilized with sucrose instead of glycylgly- cine ) . The protoplast suspension was centri- fuged , resuspended in a hypotonic solution of 50 mM Tris - maleate , pH 7.4 , 250 mM sucrose , and 5 mM MgSO4 , and ...
... protoplasts can also be stabilized with sucrose instead of glycylgly- cine ) . The protoplast suspension was centri- fuged , resuspended in a hypotonic solution of 50 mM Tris - maleate , pH 7.4 , 250 mM sucrose , and 5 mM MgSO4 , and ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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