Manual of Methods for General Bacteriology |
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Page 371
... reactions have been exploited in development of coupled en- zyme assays . These involve the coupling of a primary enzyme ( the enzyme whose activity is being measured ) system to one or two auxiliary enzymes added to the assay reaction ...
... reactions have been exploited in development of coupled en- zyme assays . These involve the coupling of a primary enzyme ( the enzyme whose activity is being measured ) system to one or two auxiliary enzymes added to the assay reaction ...
Page 373
... reaction by ad- dition of the Na2CO3 at 0 , 1 , 2 , 4 , 6 , 8 , 10 , 15 , 20 , 25 , and 30 min . Read the A420 , and determine how long the reaction remains linear with re- spect to time by plotting the A420 ( ordinate ) against time of ...
... reaction by ad- dition of the Na2CO3 at 0 , 1 , 2 , 4 , 6 , 8 , 10 , 15 , 20 , 25 , and 30 min . Read the A420 , and determine how long the reaction remains linear with re- spect to time by plotting the A420 ( ordinate ) against time of ...
Page 465
... reaction mixtures must have a greater volume . Prepare reaction mixtures in 13 by 100 mm screw - cap test tubes as follows : Labeled DNA 0.12 ml 0.60 ml Unlabeled DNA 0.88 M NaCl - 10-3 M HEPES buffer 0.60 ml 1.32 ml Total volume 1 ...
... reaction mixtures must have a greater volume . Prepare reaction mixtures in 13 by 100 mm screw - cap test tubes as follows : Labeled DNA 0.12 ml 0.60 ml Unlabeled DNA 0.88 M NaCl - 10-3 M HEPES buffer 0.60 ml 1.32 ml Total volume 1 ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract