Manual of Methods for General Bacteriology |
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Page 371
GENERAL CONSIDERATIONS 371 B D Competing side reactions and use of
coefficient of 6 . 22 + 106 cm2 ... 05 M trisodium or enzymes added to the assay
reaction mixture : tricyclohexylammonium phosphoenolpyruvate , 0 . 15 M
sodium ...
GENERAL CONSIDERATIONS 371 B D Competing side reactions and use of
coefficient of 6 . 22 + 106 cm2 ... 05 M trisodium or enzymes added to the assay
reaction mixture : tricyclohexylammonium phosphoenolpyruvate , 0 . 15 M
sodium ...
Page 373
2 . 4 . OXIDOREDUCTASES 373 Enzyme activity versus time . In duplicate ,
prepare 10 to 20 enzyme reaction mixtures complete with a suitable mass of cells
( a few preliminary trials with different dilutions of cells will generally be needed )
.
2 . 4 . OXIDOREDUCTASES 373 Enzyme activity versus time . In duplicate ,
prepare 10 to 20 enzyme reaction mixtures complete with a suitable mass of cells
( a few preliminary trials with different dilutions of cells will generally be needed )
.
Page 375
Cool for 10 min in a covered cold - water bath ( light causes a side reaction to
occur ) . Read absorbancy at 490 nm , and calculate moles of citrulline produced
by reference to a standard curve prepared in an identical fashion with L -
citrulline ...
Cool for 10 min in a covered cold - water bath ( light causes a side reaction to
occur ) . Read absorbancy at 490 nm , and calculate moles of citrulline produced
by reference to a standard curve prepared in an identical fashion with L -
citrulline ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York