Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
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Page 371
GENERAL CONSIDERATIONS 371 Competing side reactions and use of
coupled enzyme assays. ... enzymes added to the assay reaction mixture:
primary ^ auxiliary enzyme primary enzyme enzyme auxiliary enzymei auxiliary
enzyme2 D ...
GENERAL CONSIDERATIONS 371 Competing side reactions and use of
coupled enzyme assays. ... enzymes added to the assay reaction mixture:
primary ^ auxiliary enzyme primary enzyme enzyme auxiliary enzymei auxiliary
enzyme2 D ...
Page 373
18.2.4. OXIDOREDUCTASES 373 Enzyme activity versus time. In duplicate,
prepare 10 to 20 enzyme reaction mixtures complete with a suitable mass of cells
(a few preliminary trials with different dilutions of cells will generally be needed).
18.2.4. OXIDOREDUCTASES 373 Enzyme activity versus time. In duplicate,
prepare 10 to 20 enzyme reaction mixtures complete with a suitable mass of cells
(a few preliminary trials with different dilutions of cells will generally be needed).
Page 375
The reaction is started with the addition of phosphofructokinase (containing no
more than ca. 10 mU of enzyme) and is followed by measuring the decrease in
optical density at 340 nm. A blank without GTP must be included in each
experiment ...
The reaction is started with the addition of phosphofructokinase (containing no
more than ca. 10 mU of enzyme) and is followed by measuring the decrease in
optical density at 340 nm. A blank without GTP must be included in each
experiment ...
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Contents
Electron Microscopy Roger M Cole and Terry J Popkin | 34 |
Cell Fractionation Carl A Schnaitman | 52 |
GROWTH Ralph N Costilow Editor | 65 |
Copyright | |
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Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction g Distilled glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove riboflavin salts sample slide sodium solution specific staining standard sterile substrate sucrose supernatant surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
| Author | American Society for Microbiology |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › › Bacteriological technics Bacteriology Science / General Science / Life Sciences / Microbiology |
| Export Citation | BiBTeX EndNote RefMan |