Manual of Methods for General Bacteriology |
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Page 39
Many different heavy metal salts have been used as negative stains . In our
experience , ammonium molybdate is the most useful because it spreads well ,
does not react with proteins or most other components in a suspension , and can
be ...
Many different heavy metal salts have been used as negative stains . In our
experience , ammonium molybdate is the most useful because it spreads well ,
does not react with proteins or most other components in a suspension , and can
be ...
Page 93
Mineral salts . Dissolve 25 g of KH2PO4 and 25 g of K2HPO4 in distilled water ,
and make up to 250 ml . This solution is known as " salts A . ” Combine the
remaining salts as follows ( “ salts B ” ) ; 10 g of MgSO4 . 7H20 , 0 . 5 g of FeSO4•
7H20 ...
Mineral salts . Dissolve 25 g of KH2PO4 and 25 g of K2HPO4 in distilled water ,
and make up to 250 ml . This solution is known as " salts A . ” Combine the
remaining salts as follows ( “ salts B ” ) ; 10 g of MgSO4 . 7H20 , 0 . 5 g of FeSO4•
7H20 ...
Page 436
1 , 000 ml Salts solution : CaCl2 ( anhydrous ) , 0 . 2 g ; MgSO4 . 7H2O , 0 . 48 g ;
K2HPO4 , 1 . 0 g ; KH2PO4 , 1 . 0 g ; NaHCO3 , 10 . 0 g ; NaCl , 2 . 0 g . Mix the
CaCl2 and MgSO4 in 300 ml of distilled water until dissolved . Add 500 ml of ...
1 , 000 ml Salts solution : CaCl2 ( anhydrous ) , 0 . 2 g ; MgSO4 . 7H2O , 0 . 48 g ;
K2HPO4 , 1 . 0 g ; KH2PO4 , 1 . 0 g ; NaHCO3 , 10 . 0 g ; NaCl , 2 . 0 g . Mix the
CaCl2 and MgSO4 in 300 ml of distilled water until dissolved . Add 500 ml of ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |