Manual of Methods for General Bacteriology |
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Page 56
... sedimentation velocities . For ex- ample , centrifugation for 5 to 10 min at 3,000 to 5,000 × g will pellet intact bacterial cells while leaving most cell fragments in the supernatant . Cell wall fragments and large membrane struc ...
... sedimentation velocities . For ex- ample , centrifugation for 5 to 10 min at 3,000 to 5,000 × g will pellet intact bacterial cells while leaving most cell fragments in the supernatant . Cell wall fragments and large membrane struc ...
Page 60
... SEDIMENT I SUPERNATANT III ( CYTOPLASMIC MEMBRANE ) Combine with Sedi- ment II SEDIMENT II Suspend combined sediments at 10 mg of protein per ml in same buffer used for breakage Add Triton X - 100 ; fi- nal concentration , 20 mg / ml ...
... SEDIMENT I SUPERNATANT III ( CYTOPLASMIC MEMBRANE ) Combine with Sedi- ment II SEDIMENT II Suspend combined sediments at 10 mg of protein per ml in same buffer used for breakage Add Triton X - 100 ; fi- nal concentration , 20 mg / ml ...
Page 344
... sediment the lipid granules . The temperature during centrif- ugation is not important . Suspend the sediment in water , and centrifuge at 10,000 × g for 15 min . Discard the supernatant fraction , and wash the lipid granules adhering ...
... sediment the lipid granules . The temperature during centrif- ugation is not important . Suspend the sediment in water , and centrifuge at 10,000 × g for 15 min . Discard the supernatant fraction , and wash the lipid granules adhering ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract