Manual of Methods for General Bacteriology |
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Page 128
... shown , and then streak back and forth over section 3. ( E ) Incubate the dish in an inverted position as shown to prevent drops of con- densed water on lid from falling onto the agar sur- face . Section 1 will develop the heaviest ...
... shown , and then streak back and forth over section 3. ( E ) Incubate the dish in an inverted position as shown to prevent drops of con- densed water on lid from falling onto the agar sur- face . Section 1 will develop the heaviest ...
Page 379
... shown in Table 1. Incubate at 37 ° C for up to 20 min ( depending on activity of the extract ) , and stop the reaction by adding 0.1 ml of 50 % ( wt / vol ) trichloroacetic acid . Dilute a fraction ( again , depending on the activity of ...
... shown in Table 1. Incubate at 37 ° C for up to 20 min ( depending on activity of the extract ) , and stop the reaction by adding 0.1 ml of 50 % ( wt / vol ) trichloroacetic acid . Dilute a fraction ( again , depending on the activity of ...
Page 440
... shown in Table 2 to obtain the appropriate pH value . The mixture ( 0.2 M phosphate buffer ) can be further diluted to give the desired molarity . Also see part 6.1.2 . 20.4.17 . Phosphate - Buffered Saline ( PBS ) 10x Stock solution ...
... shown in Table 2 to obtain the appropriate pH value . The mixture ( 0.2 M phosphate buffer ) can be further diluted to give the desired molarity . Also see part 6.1.2 . 20.4.17 . Phosphate - Buffered Saline ( PBS ) 10x Stock solution ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract