Manual of Methods for General Bacteriology |
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Page 30
by heating loosely packed slides at 400°C for 20 min in a muffle furnace .
Remove the slides after cooling , and use immediately or store in dust - free
containers . ... Immerse and withdraw the slide several times in xylene , and blot
dry . 4 .
by heating loosely packed slides at 400°C for 20 min in a muffle furnace .
Remove the slides after cooling , and use immediately or store in dust - free
containers . ... Immerse and withdraw the slide several times in xylene , and blot
dry . 4 .
Page 280
The slides should be acid cleaned , further washed with a detergent solution ,
and then thoroughly rinsed with distilled water . ... Sprinkle a small amount of fine
talcum powder about half way between the slide and the front of the dish .
The slides should be acid cleaned , further washed with a detergent solution ,
and then thoroughly rinsed with distilled water . ... Sprinkle a small amount of fine
talcum powder about half way between the slide and the front of the dish .
Page 430
Rock the slide in a circular fashion for 1 min to further mix the cells and liquid ,
being careful not to spill over the boundary of the ring . Examine the slide by eye ;
for best visibility hold the slide near a bright light and view against a dark ...
Rock the slide in a circular fashion for 1 min to further mix the cells and liquid ,
being careful not to spill over the boundary of the ring . Examine the slide by eye ;
for best visibility hold the slide near a bright light and view against a dark ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York