Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
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Page 30
by heating loosely packed slides at 400 °C for 20 min in a muffle furnace.
Remove the slides after cooling, and ... Place 1 ml of the dye solution on the slide
without allowing any to flow over the wax lines. Leave for 7 to 15 min; the "best"
time ...
by heating loosely packed slides at 400 °C for 20 min in a muffle furnace.
Remove the slides after cooling, and ... Place 1 ml of the dye solution on the slide
without allowing any to flow over the wax lines. Leave for 7 to 15 min; the "best"
time ...
Page 31
Add 10 ml of 2 N hydrochloric acid and 4 g of potassium metabisulfite to the
filtrate. Stopper tightly and allow to stand for 12 h in a cool, dark place. Add about
10 ml of 2 N hydrochloric acid until the reagent when dried on a glass slide does
not ...
Add 10 ml of 2 N hydrochloric acid and 4 g of potassium metabisulfite to the
filtrate. Stopper tightly and allow to stand for 12 h in a cool, dark place. Add about
10 ml of 2 N hydrochloric acid until the reagent when dried on a glass slide does
not ...
Page 280
The slides should be acid cleaned, further washed with a detergent solution, and
then thoroughly rinsed with distilled water. The trough ... Sprinkle a small amount
of fine talcum powder about half way between the slide and the front of the dish.
The slides should be acid cleaned, further washed with a detergent solution, and
then thoroughly rinsed with distilled water. The trough ... Sprinkle a small amount
of fine talcum powder about half way between the slide and the front of the dish.
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Contents
Electron Microscopy Roger M Cole and Terry J Popkin | 34 |
Cell Fractionation Carl A Schnaitman | 52 |
GROWTH Ralph N Costilow Editor | 65 |
Copyright | |
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Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction g Distilled glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove riboflavin salts sample slide sodium solution specific staining standard sterile substrate sucrose supernatant surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
| Author | American Society for Microbiology |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › › Bacteriological technics Bacteriology Science / General Science / Life Sciences / Microbiology |
| Export Citation | BiBTeX EndNote RefMan |