Manual of Methods for General Bacteriology |
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Page 31
3 . SPECIAL TECHNIQUES : RICKETTSIA 31 boiling distilled water , cool to 50°C
, and filter Fontana method for staining spirochetes . through paper . Add 10 ml of
2 N hydrochloric Solution A ( fixative ) : acid and 4 g of potassium metabisulfite ...
3 . SPECIAL TECHNIQUES : RICKETTSIA 31 boiling distilled water , cool to 50°C
, and filter Fontana method for staining spirochetes . through paper . Add 10 ml of
2 N hydrochloric Solution A ( fixative ) : acid and 4 g of potassium metabisulfite ...
Page 438
NAD solution : Nicotinamide adenine dinucleotide ( NAD , catalog no . N - 7004 ;
Sigma ... Solution should be freshly prepared . 20 . 4 . 5 . ... Add 20 pellets of
NaOH with stirring until the pellets are dissolved and the fumaric acid is in
solution .
NAD solution : Nicotinamide adenine dinucleotide ( NAD , catalog no . N - 7004 ;
Sigma ... Solution should be freshly prepared . 20 . 4 . 5 . ... Add 20 pellets of
NaOH with stirring until the pellets are dissolved and the fumaric acid is in
solution .
Page 439
5 % agar solution , using 10 ml of fat per 90 ml of agar . Sterilize ... 5 ml Dissolve
the ONPG in the distilled water at 37°C . Add the sodium phosphate solution . ...
Mix equal volumes of solutions A and B just before use to make the test reagent .
5 % agar solution , using 10 ml of fat per 90 ml of agar . Sterilize ... 5 ml Dissolve
the ONPG in the distilled water at 37°C . Add the sodium phosphate solution . ...
Mix equal volumes of solutions A and B just before use to make the test reagent .
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |