Manual of Methods for General Bacteriology |
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Page 31
... solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt / vol ) aqueous solution of ...
... solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt / vol ) aqueous solution of ...
Page 438
... solution : Nicotinamide adenine dinucleotide ( NAD , catalog no . N - 7004 ; Sigma Chemical Co. ) Distilled water 0.160 g 8.0 ml Combine the ingredients and dilute to 100 ml with n - propanol . 20.4.5 . Ehrlich Reagent p ...
... solution : Nicotinamide adenine dinucleotide ( NAD , catalog no . N - 7004 ; Sigma Chemical Co. ) Distilled water 0.160 g 8.0 ml Combine the ingredients and dilute to 100 ml with n - propanol . 20.4.5 . Ehrlich Reagent p ...
Page 439
... Solution A ( see Caution ) : N- ( 1 - Naphthyl ) -ethylenediamine dihydrochloride ( Sigma Chemi- cal Co. ) HCl , 1.5 N solution 0.02 g 100 ml Dissolve by gentle heating in a fume hood . Solution B : Sulfanilic acid HCl , 1.5 N solution ...
... Solution A ( see Caution ) : N- ( 1 - Naphthyl ) -ethylenediamine dihydrochloride ( Sigma Chemi- cal Co. ) HCl , 1.5 N solution 0.02 g 100 ml Dissolve by gentle heating in a fume hood . Solution B : Sulfanilic acid HCl , 1.5 N solution ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract