Manual of Methods for General Bacteriology |
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Page 18
If necessary , slide cultures can be fixed for staining procedures and even
prepared for electron microscopy , but such flat embeddings ( 2 , 7 ) need
considerable skill in handling and sectioning . The cover slip , with undisturbed
culture and ...
If necessary , slide cultures can be fixed for staining procedures and even
prepared for electron microscopy , but such flat embeddings ( 2 , 7 ) need
considerable skill in handling and sectioning . The cover slip , with undisturbed
culture and ...
Page 26
Solution B for staining reagent : Ammonium oxalate , 0 . 8 g Distilled water , 80 ml
Mix A and B to obtain the crystal violet staining reagent . Store for 24 h and filter
through paper before using . Mordant : Iodine , 1 . 0 g Potassium iodide , 2 .
Solution B for staining reagent : Ammonium oxalate , 0 . 8 g Distilled water , 80 ml
Mix A and B to obtain the crystal violet staining reagent . Store for 24 h and filter
through paper before using . Mordant : Iodine , 1 . 0 g Potassium iodide , 2 .
Page 322
bumin ) . containing 1 ug of protein is normally visible rinse the gels several times
in 10 % ( wt / vol ) triafter staining with Coomassie blue . Care must chloroacetic
acid - 33 % ( vol / vol ) methanol in wabe taken that some of the sample is not ...
bumin ) . containing 1 ug of protein is normally visible rinse the gels several times
in 10 % ( wt / vol ) triafter staining with Coomassie blue . Care must chloroacetic
acid - 33 % ( vol / vol ) methanol in wabe taken that some of the sample is not ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York