Manual of Methods for General Bacteriology |
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Page 127
... sterile water and agitate it for several minutes . Add 1 ml of sterile water to each of six sterile petri dishes . Transfer the nodule to the first dish and crush it with sterile forceps . Mix the exudate with the water . Transfer one ...
... sterile water and agitate it for several minutes . Add 1 ml of sterile water to each of six sterile petri dishes . Transfer the nodule to the first dish and crush it with sterile forceps . Mix the exudate with the water . Transfer one ...
Page 209
... sterile medicinal - grade mineral oil ( paraffin oil of spe- cific gravity of 0.865 to 0.890 is satisfactory ) . Contamination of cultures when this method is used is often due to improperly sterilized mineral oil . Sterilize the oil by ...
... sterile medicinal - grade mineral oil ( paraffin oil of spe- cific gravity of 0.865 to 0.890 is satisfactory ) . Contamination of cultures when this method is used is often due to improperly sterilized mineral oil . Sterilize the oil by ...
Page 263
... sterile 50x salts to about 960 ml of sterile distilled or deionized water so that , upon addi- tion of sterile carbohydrate and other required supplements ( see below ) , the final volume will be 1 liter . To prepare 1x VB minimal agar ...
... sterile 50x salts to about 960 ml of sterile distilled or deionized water so that , upon addi- tion of sterile carbohydrate and other required supplements ( see below ) , the final volume will be 1 liter . To prepare 1x VB minimal agar ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract