Manual of Methods for General Bacteriology |
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Page 56
... sucrose , and 0.5 mg of lysozyme per ml is placed in a flask and shell frozen by swirling in a dry ice - acetone bath . The flask is then thawed in warm water until the ice is just melted , and the contents are poured into 20 volumes of ...
... sucrose , and 0.5 mg of lysozyme per ml is placed in a flask and shell frozen by swirling in a dry ice - acetone bath . The flask is then thawed in warm water until the ice is just melted , and the contents are poured into 20 volumes of ...
Page 57
... Sucrose Gradients Sucrose concentrations in gradients are re- ported in the literature in several ways : density , molar concentration of sucrose , percent sucrose by weight ( % , wt / wt ) , and percent sucrose per unit volume ( % , wt ...
... Sucrose Gradients Sucrose concentrations in gradients are re- ported in the literature in several ways : density , molar concentration of sucrose , percent sucrose by weight ( % , wt / wt ) , and percent sucrose per unit volume ( % , wt ...
Page 167
... sucrose , colloidal silica sol , or other appropriate material as dictated by the cell system . To prepare an exponential sucrose gradient in a discontinuous manner , layer sterile ice - cold solutions of increasing sucrose concen ...
... sucrose , colloidal silica sol , or other appropriate material as dictated by the cell system . To prepare an exponential sucrose gradient in a discontinuous manner , layer sterile ice - cold solutions of increasing sucrose concen ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract