Using Antibodies: A Laboratory Manual, Volume 286 |
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Page 4
... bind with sufficient affinity that they can be used as specific reagents to study their cognate antigens . Serum contains a collection of antibodies that bind to the antigen on different epitopes The antibody - binding site on an ...
... bind with sufficient affinity that they can be used as specific reagents to study their cognate antigens . Serum contains a collection of antibodies that bind to the antigen on different epitopes The antibody - binding site on an ...
Page 5
... binding and cell - binding regions antibodies Calf antibodies Antibodies are secreted glycoproteins that perform two key functional roles . To be ef- fective , they must have regions that bind to foreign antigens and to specialized ...
... binding and cell - binding regions antibodies Calf antibodies Antibodies are secreted glycoproteins that perform two key functional roles . To be ef- fective , they must have regions that bind to foreign antigens and to specialized ...
Page 6
... binding region and the immune cell - binding region is called the hinge . This segment allows lateral and rotational movement of the two antigen - binding domains . In practice , this movement allows the binding regions freedom to ...
... binding region and the immune cell - binding region is called the hinge . This segment allows lateral and rotational movement of the two antigen - binding domains . In practice , this movement allows the binding regions freedom to ...
Page 8
... binding sites , IgMs have 10 identical antigen - binding sites . The sites for association between the different Y units are also found in the Fc region . Table 1.2 summarizes many of the properties of these antibody classes . Although ...
... binding sites , IgMs have 10 identical antigen - binding sites . The sites for association between the different Y units are also found in the Fc region . Table 1.2 summarizes many of the properties of these antibody classes . Although ...
Page 10
... binding site . The heterogeneity of the variable regions provides the structural ba- sis for the large repertoire of binding sites used by an animal to mount an effective im- mune response . Perhaps not surprisingly , the sequence ...
... binding site . The heterogeneity of the variable regions provides the structural ba- sis for the large repertoire of binding sites used by an animal to mount an effective im- mune response . Perhaps not surprisingly , the sequence ...
Contents
43 | |
Immunostaining | 152 |
allows the partial | 222 |
Immunoblotting | 242 |
provides a reliable | 254 |
method to check | 268 |
purification takes | 312 |
Tagging Proteins | 345 |
Determining | 380 |
contains | 423 |
Protein Techniques | 430 |
covered in Appendix | 454 |
Other editions - View all
Using Antibodies: A Laboratory Manual, Volume 286 Edward Harlow,David Lane No preview available - 1999 |
Common terms and phrases
affinity allow amino acids anti antibody binding antibody solution antibody-antigen antigen Appendix ascites assay background problems beads biotin blot Caution cell staining chain chemical chloride column commercial commonly complex coverslips cross-reactions denatured detection methods detergents determine dilutions electrophoresis elution embryos enzyme epitopes fixation fixed fluorochromes gene hybridoma immune immunoaffinity purification immunoblotting immunoglobulin immunoprecipitation immunostaining Incubate interactions labeled antibody labeled secondary reagent lysate lysis buffer membrane methanol mg/ml microscope minutes at room molecular weight molecules monoclonal antibodies Needed solutions nitrocellulose nonspecific normally paraformaldehyde peptide polyclonal antibodies polypeptide preclearing primary antibody procedure protease protein antigens protein G protocols purified antibodies reaction recommend remove room temperature samples secondary antibodies sensitivity serum signal slides sodium azide specific antibody specimen step stored streptavidin structure substrate supernatant tein tibody tion tissue tissue-culture transfer Tris pH tube volume Wash Wear appropriate gloves µg/ml
Popular passages
Page 29 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
Page 422 - Davis, BJ (1964) Disc electrophoresis — II. Method and application to human serum proteins.
Page 333 - K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gin; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Тут.
Page 10 - Segal, DM, Padlan, EA, Cohen, GH, Rudikoff, S., Potter, M., and Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
Page 422 - Laskey, RA and Mills, AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Page 422 - Bonner, WM, and Laskey, RA (1974) A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels.
Page 30 - Amit AG, Mariuzza RA, Phillips SEV, and Poljak RJ 1986. Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution.
Page 13 - Alt, FW/ Yancopoulos, GD/ Blackwell/ TK, Wood/ C./ Thomas/ E., Boss/ M./ Coffman/. R./ Rosenberg/ N., Tonegawa/ S. and Baltimore/ D.
Page 13 - Activity of multiple light 18 chain genes in murine myeloma cells producing a single, functional light chain. Cell 21: 1-12.