Using Antibodies: A Laboratory Manual, Volume 286 |
From inside the book
Results 1-5 of 16
Page 5
... bound by an antibody or a T - cell receptor . In the process of developing an immune response , the terms " immunogen " and " antigen " can be considered identical , because one essential step in inducing immunity is that the molecule ...
... bound by an antibody or a T - cell receptor . In the process of developing an immune response , the terms " immunogen " and " antigen " can be considered identical , because one essential step in inducing immunity is that the molecule ...
Page 13
... bound with antigen to this FCR promotes phagocytosis when bound to macrophages and granulocytes or antibody - dependent cellular cyto- toxicity when bound to lymphocytes and natural killer cells . There are important do- mains for the ...
... bound with antigen to this FCR promotes phagocytosis when bound to macrophages and granulocytes or antibody - dependent cellular cyto- toxicity when bound to lymphocytes and natural killer cells . There are important do- mains for the ...
Page 14
... bound to an Fab fragment . Nature 359 : 752-754 . Edelman G.M. 1959. Dissociation of gamma globulin . J. Am . Chem . Soc . 81 : 3155-3156 . Edelman G.M. , Cunningham B.A. , Gall W.E. , Gottlieb P.D. , Rutishauser U. , and Waxdal M.J. ...
... bound to an Fab fragment . Nature 359 : 752-754 . Edelman G.M. 1959. Dissociation of gamma globulin . J. Am . Chem . Soc . 81 : 3155-3156 . Edelman G.M. , Cunningham B.A. , Gall W.E. , Gottlieb P.D. , Rutishauser U. , and Waxdal M.J. ...
Page 17
... Bound- aries of somatic mutation in rearranged immunoglobulin genes : 5 ' boundary is near the promoter , and 3 ' boundary is about 1 kb from V ( D ) J gene . J. Exp . Med . 172 : 1717-1727 . Maizels N. 1995. Somatic hypermutation : How ...
... Bound- aries of somatic mutation in rearranged immunoglobulin genes : 5 ' boundary is near the promoter , and 3 ' boundary is about 1 kb from V ( D ) J gene . J. Exp . Med . 172 : 1717-1727 . Maizels N. 1995. Somatic hypermutation : How ...
Page 21
... bound to the side of the active site cavity on the enzyme . The en- zyme active centre is facing the viewer , below and to the left [ now right ] of the antibody binding site . To understand how to use antibodies as an effective ...
... bound to the side of the active site cavity on the enzyme . The en- zyme active centre is facing the viewer , below and to the left [ now right ] of the antibody binding site . To understand how to use antibodies as an effective ...
Contents
43 | |
Immunostaining | 152 |
allows the partial | 222 |
Immunoblotting | 242 |
provides a reliable | 254 |
method to check | 268 |
purification takes | 312 |
Tagging Proteins | 345 |
Determining | 380 |
contains | 423 |
Protein Techniques | 430 |
covered in Appendix | 454 |
Other editions - View all
Using Antibodies: A Laboratory Manual, Volume 286 Edward Harlow,David Lane No preview available - 1999 |
Common terms and phrases
affinity allow amino acids anti antibody binding antibody solution antibody-antigen antigen Appendix ascites assay background problems beads biotin blot Caution cell staining chain chemical chloride column commercial commonly complex coverslips cross-reactions denatured detection methods detergents determine dilutions electrophoresis elution embryos enzyme epitopes fixation fixed fluorochromes gene hybridoma immune immunoaffinity purification immunoblotting immunoglobulin immunoprecipitation immunostaining Incubate interactions labeled antibody labeled secondary reagent lysate lysis buffer membrane methanol mg/ml microscope minutes at room molecular weight molecules monoclonal antibodies Needed solutions nitrocellulose nonspecific normally paraformaldehyde peptide polyclonal antibodies polypeptide preclearing primary antibody procedure protease protein antigens protein G protocols purified antibodies reaction recommend remove room temperature samples secondary antibodies sensitivity serum signal slides sodium azide specific antibody specimen step stored streptavidin structure substrate supernatant tein tibody tion tissue tissue-culture transfer Tris pH tube volume Wash Wear appropriate gloves µg/ml
Popular passages
Page 29 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
Page 422 - Davis, BJ (1964) Disc electrophoresis — II. Method and application to human serum proteins.
Page 333 - K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gin; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Тут.
Page 10 - Segal, DM, Padlan, EA, Cohen, GH, Rudikoff, S., Potter, M., and Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
Page 422 - Laskey, RA and Mills, AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Page 422 - Bonner, WM, and Laskey, RA (1974) A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels.
Page 30 - Amit AG, Mariuzza RA, Phillips SEV, and Poljak RJ 1986. Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution.
Page 13 - Alt, FW/ Yancopoulos, GD/ Blackwell/ TK, Wood/ C./ Thomas/ E., Boss/ M./ Coffman/. R./ Rosenberg/ N., Tonegawa/ S. and Baltimore/ D.
Page 13 - Activity of multiple light 18 chain genes in murine myeloma cells producing a single, functional light chain. Cell 21: 1-12.