Using Antibodies: A Laboratory Manual, Volume 286 |
From inside the book
Results 1-5 of 15
Page vii
... multiple steps over several days as well as extensive knowledge of the architecture of the tissues being studied . These procedures demand methods to preserve the structure of tissues , which unfortunately are often damaging to the ...
... multiple steps over several days as well as extensive knowledge of the architecture of the tissues being studied . These procedures demand methods to preserve the structure of tissues , which unfortunately are often damaging to the ...
Page 10
... Multiple genetic mechanisms provide immense diversity at the antigen - binding site For an animal to be able to respond to a wide variety of different and previously un- encountered antigens , it must have at its call an extremely large ...
... Multiple genetic mechanisms provide immense diversity at the antigen - binding site For an animal to be able to respond to a wide variety of different and previously un- encountered antigens , it must have at its call an extremely large ...
Page 11
... multiple coding segments , each specifying a portion of the antigen - binding site ; ( 2 ) a slightly inaccurate homologous recombination pro- cess that both realigns the various coding regions to create the final antibody sequence and ...
... multiple coding segments , each specifying a portion of the antigen - binding site ; ( 2 ) a slightly inaccurate homologous recombination pro- cess that both realigns the various coding regions to create the final antibody sequence and ...
Page 16
... Multiple V , D , and J regions as a source of diversity Chuchana P. , Blancher A. , Brockly F. , Alexan- dre D. , Lefranc G. , and Lefranc M.P. 1990 . Definition of the human immunoglobulin variable lambda ( IGLV ) gene subgroups . Eur ...
... Multiple V , D , and J regions as a source of diversity Chuchana P. , Blancher A. , Brockly F. , Alexan- dre D. , Lefranc G. , and Lefranc M.P. 1990 . Definition of the human immunoglobulin variable lambda ( IGLV ) gene subgroups . Eur ...
Page 18
... 85 : 107-113 . Sequence of VJ / VDJ joining and allelic exclusion Alt F.W. , Enea V. , Bothwell A.L.M. , and Balti- more D. 1980. Activity of multiple light chain genes in murine myeloma cells pro- ducing a single 18.
... 85 : 107-113 . Sequence of VJ / VDJ joining and allelic exclusion Alt F.W. , Enea V. , Bothwell A.L.M. , and Balti- more D. 1980. Activity of multiple light chain genes in murine myeloma cells pro- ducing a single 18.
Contents
43 | |
Immunostaining | 152 |
allows the partial | 222 |
Immunoblotting | 242 |
provides a reliable | 254 |
method to check | 268 |
purification takes | 312 |
Tagging Proteins | 345 |
Determining | 380 |
contains | 423 |
Protein Techniques | 430 |
covered in Appendix | 454 |
Other editions - View all
Using Antibodies: A Laboratory Manual, Volume 286 Edward Harlow,David Lane No preview available - 1999 |
Common terms and phrases
affinity allow amino acids anti antibody binding antibody solution antibody-antigen antigen Appendix ascites assay background problems beads biotin blot Caution cell staining chain chemical chloride column commercial commonly complex coverslips cross-reactions denatured detection methods detergents determine dilutions electrophoresis elution embryos enzyme epitopes fixation fixed fluorochromes gene hybridoma immune immunoaffinity purification immunoblotting immunoglobulin immunoprecipitation immunostaining Incubate interactions labeled antibody labeled secondary reagent lysate lysis buffer membrane methanol mg/ml microscope minutes at room molecular weight molecules monoclonal antibodies Needed solutions nitrocellulose nonspecific normally paraformaldehyde peptide polyclonal antibodies polypeptide preclearing primary antibody procedure protease protein antigens protein G protocols purified antibodies reaction recommend remove room temperature samples secondary antibodies sensitivity serum signal slides sodium azide specific antibody specimen step stored streptavidin structure substrate supernatant tein tibody tion tissue tissue-culture transfer Tris pH tube volume Wash Wear appropriate gloves µg/ml
Popular passages
Page 29 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
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Page 10 - Segal, DM, Padlan, EA, Cohen, GH, Rudikoff, S., Potter, M., and Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
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Page 13 - Alt, FW/ Yancopoulos, GD/ Blackwell/ TK, Wood/ C./ Thomas/ E., Boss/ M./ Coffman/. R./ Rosenberg/ N., Tonegawa/ S. and Baltimore/ D.
Page 13 - Activity of multiple light 18 chain genes in murine myeloma cells producing a single, functional light chain. Cell 21: 1-12.