Using Antibodies: A Laboratory Manual, Volume 286 |
From inside the book
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Page vii
... structure of tissues , which unfortunately are often damaging to the antigens . 151 7 Immunoprecipitation Immunoprecipitation allows the partial purification of antigens , normally proteins , from complex mixtures of soluble molecules ...
... structure of tissues , which unfortunately are often damaging to the antigens . 151 7 Immunoprecipitation Immunoprecipitation allows the partial purification of antigens , normally proteins , from complex mixtures of soluble molecules ...
Page 1
... structure and func- tion to its current detailed level . As a result , we probably know as much or more about the biology of antibodies than we do for any other protein in mammalian biol- ogy . This information base has been developed ...
... structure and func- tion to its current detailed level . As a result , we probably know as much or more about the biology of antibodies than we do for any other protein in mammalian biol- ogy . This information base has been developed ...
Page 2
... mostly through phagocytosis by macrophages . This antibody response is one of the key mechanisms that a host or- ganism uses to protect against the action of foreign molecules or organisms . 8 2 !! 9 ΤΟ 1 Y Antibody Structure and.
... mostly through phagocytosis by macrophages . This antibody response is one of the key mechanisms that a host or- ganism uses to protect against the action of foreign molecules or organisms . 8 2 !! 9 ΤΟ 1 Y Antibody Structure and.
Page 3
A Laboratory Manual Edward Harlow, David Lane. 8 2 !! 9 ΤΟ 1 Y Antibody Structure and Function Antibodies are useful reagents that can bind with high affinity. 3.
A Laboratory Manual Edward Harlow, David Lane. 8 2 !! 9 ΤΟ 1 Y Antibody Structure and Function Antibodies are useful reagents that can bind with high affinity. 3.
Page 4
... from the tumor - bearing mice . This gives a very high titer preparation of monoclonal antibodies . For specialized needs , the mono- What is the difference between an antigen and an immunogen. 4 Antibody Structure and Function.
... from the tumor - bearing mice . This gives a very high titer preparation of monoclonal antibodies . For specialized needs , the mono- What is the difference between an antigen and an immunogen. 4 Antibody Structure and Function.
Contents
43 | |
Immunostaining | 152 |
allows the partial | 222 |
Immunoblotting | 242 |
provides a reliable | 254 |
method to check | 268 |
purification takes | 312 |
Tagging Proteins | 345 |
Determining | 380 |
contains | 423 |
Protein Techniques | 430 |
covered in Appendix | 454 |
Other editions - View all
Using Antibodies: A Laboratory Manual, Volume 286 Edward Harlow,David Lane No preview available - 1999 |
Common terms and phrases
affinity allow amino acids anti antibody binding antibody solution antibody-antigen antigen Appendix ascites assay background problems beads biotin blot Caution cell staining chain chemical chloride column commercial commonly complex coverslips cross-reactions denatured detection methods detergents determine dilutions electrophoresis elution embryos enzyme epitopes fixation fixed fluorochromes gene hybridoma immune immunoaffinity purification immunoblotting immunoglobulin immunoprecipitation immunostaining Incubate interactions labeled antibody labeled secondary reagent lysate lysis buffer membrane methanol mg/ml microscope minutes at room molecular weight molecules monoclonal antibodies Needed solutions nitrocellulose nonspecific normally paraformaldehyde peptide polyclonal antibodies polypeptide preclearing primary antibody procedure protease protein antigens protein G protocols purified antibodies reaction recommend remove room temperature samples secondary antibodies sensitivity serum signal slides sodium azide specific antibody specimen step stored streptavidin structure substrate supernatant tein tibody tion tissue tissue-culture transfer Tris pH tube volume Wash Wear appropriate gloves µg/ml
Popular passages
Page 29 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
Page 422 - Davis, BJ (1964) Disc electrophoresis — II. Method and application to human serum proteins.
Page 333 - K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gin; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Тут.
Page 10 - Segal, DM, Padlan, EA, Cohen, GH, Rudikoff, S., Potter, M., and Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
Page 422 - Laskey, RA and Mills, AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Page 422 - Bonner, WM, and Laskey, RA (1974) A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels.
Page 30 - Amit AG, Mariuzza RA, Phillips SEV, and Poljak RJ 1986. Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution.
Page 13 - Alt, FW/ Yancopoulos, GD/ Blackwell/ TK, Wood/ C./ Thomas/ E., Boss/ M./ Coffman/. R./ Rosenberg/ N., Tonegawa/ S. and Baltimore/ D.
Page 13 - Activity of multiple light 18 chain genes in murine myeloma cells producing a single, functional light chain. Cell 21: 1-12.