Using Antibodies: A Laboratory Manual, Volume 286 |
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Page 4
... techniques . They are normally used as crude serum samples and in this form are fine for most purposes . For some more specialized techniques , the antibodies need to be purified prior to use . Polyclonal antibodies can be purified by ...
... techniques . They are normally used as crude serum samples and in this form are fine for most purposes . For some more specialized techniques , the antibodies need to be purified prior to use . Polyclonal antibodies can be purified by ...
Page 13
... techniques , the constant domains also provide an important site for interaction with key secondary reagents . Since these regions are distal to the antigen - combining sites , they offer a site to interact with the antibody without ...
... techniques , the constant domains also provide an important site for interaction with key secondary reagents . Since these regions are distal to the antigen - combining sites , they offer a site to interact with the antibody without ...
Page 22
... techniques . This chapter discusses the properties of the antibody - anti- gen interaction and is divided into three sections . The first summarizes the structure of the antibody - antigen bonds , the second covers the strength of these ...
... techniques . This chapter discusses the properties of the antibody - anti- gen interaction and is divided into three sections . The first summarizes the structure of the antibody - antigen bonds , the second covers the strength of these ...
Page 24
... techniques have delineated the region of the anti- body molecule that is involved in antigen binding , the region of the antigen molecule that interacts with the antibody , and the molecular basis for antibody specificity . The antigen ...
... techniques have delineated the region of the anti- body molecule that is involved in antigen binding , the region of the antigen molecule that interacts with the antibody , and the molecular basis for antibody specificity . The antigen ...
Page 28
... techniques . This is due not only to their higher capacity , but also to the sta- bility of the complex . For example , the half - time for dissociation of an antibody binding to a small protein antigen with high affinity is 30 minutes ...
... techniques . This is due not only to their higher capacity , but also to the sta- bility of the complex . For example , the half - time for dissociation of an antibody binding to a small protein antigen with high affinity is 30 minutes ...
Contents
43 | |
Immunostaining | 152 |
allows the partial | 222 |
Immunoblotting | 242 |
provides a reliable | 254 |
method to check | 268 |
purification takes | 312 |
Tagging Proteins | 345 |
Determining | 380 |
contains | 423 |
Protein Techniques | 430 |
covered in Appendix | 454 |
Other editions - View all
Using Antibodies: A Laboratory Manual, Volume 286 Edward Harlow,David Lane No preview available - 1999 |
Common terms and phrases
affinity allow amino acids anti antibody binding antibody solution antibody-antigen antigen Appendix ascites assay background problems beads biotin blot Caution cell staining chain chemical chloride column commercial commonly complex coverslips cross-reactions denatured detection methods detergents determine dilutions electrophoresis elution embryos enzyme epitopes fixation fixed fluorochromes gene hybridoma immune immunoaffinity purification immunoblotting immunoglobulin immunoprecipitation immunostaining Incubate interactions labeled antibody labeled secondary reagent lysate lysis buffer membrane methanol mg/ml microscope minutes at room molecular weight molecules monoclonal antibodies Needed solutions nitrocellulose nonspecific normally paraformaldehyde peptide polyclonal antibodies polypeptide preclearing primary antibody procedure protease protein antigens protein G protocols purified antibodies reaction recommend remove room temperature samples secondary antibodies sensitivity serum signal slides sodium azide specific antibody specimen step stored streptavidin structure substrate supernatant tein tibody tion tissue tissue-culture transfer Tris pH tube volume Wash Wear appropriate gloves µg/ml
Popular passages
Page 29 - Benjamin DC, Berzofsky JA, East IJ, Gurd, FRN, Hannum C., Leach SJ, Margoliash E., Michael JG, Miller A., Prager EM, Reichlin M., Sercarz EE, Smith-Gill SJ, Todd PE, and Wilson AC 1984. The antigenic structure of proteins: A reappraisal. Annu. Rev. Immunol. 2: 67-101.
Page 422 - Davis, BJ (1964) Disc electrophoresis — II. Method and application to human serum proteins.
Page 333 - K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gin; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Тут.
Page 10 - Segal, DM, Padlan, EA, Cohen, GH, Rudikoff, S., Potter, M., and Davies, D. R. (1974) The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc. Natl. Acad. Sci. USA 71, 4298^302.
Page 422 - Laskey, RA and Mills, AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Page 422 - Bonner, WM, and Laskey, RA (1974) A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels.
Page 30 - Amit AG, Mariuzza RA, Phillips SEV, and Poljak RJ 1986. Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution.
Page 13 - Alt, FW/ Yancopoulos, GD/ Blackwell/ TK, Wood/ C./ Thomas/ E., Boss/ M./ Coffman/. R./ Rosenberg/ N., Tonegawa/ S. and Baltimore/ D.
Page 13 - Activity of multiple light 18 chain genes in murine myeloma cells producing a single, functional light chain. Cell 21: 1-12.