Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 209
... Denaturation of Myoglobin Kirste et al . ( 1969 ) studied the changes in conformation of metmyo- globin caused by changes in pH . They found that myoglobin was stable in aqueous solution in the absence of salt between pH 7 and pH 4.6 ...
... Denaturation of Myoglobin Kirste et al . ( 1969 ) studied the changes in conformation of metmyo- globin caused by changes in pH . They found that myoglobin was stable in aqueous solution in the absence of salt between pH 7 and pH 4.6 ...
Page 210
... denaturation in 6 M urea solu- tion . Echols and Anderegg , too , observed that during the denaturation the radius of gyration increased by 27 % from 33.5 A to 46 A ; urea caused a partial unfolding of the molecule by splitting hydrogen ...
... denaturation in 6 M urea solu- tion . Echols and Anderegg , too , observed that during the denaturation the radius of gyration increased by 27 % from 33.5 A to 46 A ; urea caused a partial unfolding of the molecule by splitting hydrogen ...
Page 535
... denatured proteins are substantially different from those of poly - L - lysine ( or poly - L- glutamate ) in the random - coil forms . This , of course , raises the signifi- cant quandary of the appropriate rotatory parameters for ...
... denatured proteins are substantially different from those of poly - L - lysine ( or poly - L- glutamate ) in the random - coil forms . This , of course , raises the signifi- cant quandary of the appropriate rotatory parameters for ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein component protein solution radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero