Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 17
... density measurement can be used to fix the composition of the mixture with very little uncertainty . If density - composition tables or relations are already available at a desired temperature for a particular solute in water , a highly ...
... density measurement can be used to fix the composition of the mixture with very little uncertainty . If density - composition tables or relations are already available at a desired temperature for a particular solute in water , a highly ...
Page 35
... density of an aqueous solution is increased by about 0.003 gm / ml ( the density increment ) for each 1 % increase in the concentration of a protein . ( Note : salts , buffer ions , and many other solutes in relatively large amounts in ...
... density of an aqueous solution is increased by about 0.003 gm / ml ( the density increment ) for each 1 % increase in the concentration of a protein . ( Note : salts , buffer ions , and many other solutes in relatively large amounts in ...
Page 157
... density is negligible at small angles , it causes some variation in the scattering function at large angles ... density . By measuring pro- teins in solvents of different electron densities , the whole scattering function can be split ...
... density is negligible at small angles , it causes some variation in the scattering function at large angles ... density . By measuring pro- teins in solvents of different electron densities , the whole scattering function can be split ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein component protein solution radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero