Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 19
... derived density P'D for the solvent components in the protein solution after c2 is measured . The derived density is obtained by subtracting the number of grams per milliliter of protein from the observed density ( giving c1 + c ) and ...
... derived density P'D for the solvent components in the protein solution after c2 is measured . The derived density is obtained by subtracting the number of grams per milliliter of protein from the observed density ( giving c1 + c ) and ...
Page 22
... derived from density measurements is dis- cussed in Section IV , B . The volume change can be obtained indirectly from the effect of pressure on the equilibrium constant of a reaction . AV = - RT d In K dP ( 30 ) where K is the ...
... derived from density measurements is dis- cussed in Section IV , B . The volume change can be obtained indirectly from the effect of pressure on the equilibrium constant of a reaction . AV = - RT d In K dP ( 30 ) where K is the ...
Page 53
... derived in Eqs . ( 42 ) can be different , in theory , from the corresponding values seen when Eqs . ( 42 ) are derived with the use of p2 as was done in Eqs . ( 43 ) . δι = - - 81 [ c1 c'1 ( 1 17. DENSITY AND VOLUME MEASUREMENTS 53.
... derived in Eqs . ( 42 ) can be different , in theory , from the corresponding values seen when Eqs . ( 42 ) are derived with the use of p2 as was done in Eqs . ( 43 ) . δι = - - 81 [ c1 c'1 ( 1 17. DENSITY AND VOLUME MEASUREMENTS 53.
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein component protein solution radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero