Physical Principles and Techniques of Protein Chemistry, Part 3Sydney J. Leach |
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Page 117
... pressure method . The measurement of a pressure difference per se is not even necessary . Hill ( 1959 ) has discussed the possibility of measuring very small osmotic pressures in terms of an electric or magnetic field . Ogston and Wells ...
... pressure method . The measurement of a pressure difference per se is not even necessary . Hill ( 1959 ) has discussed the possibility of measuring very small osmotic pressures in terms of an electric or magnetic field . Ogston and Wells ...
Page 124
... pressure ( usually in terms of h ) both above and below the equilibrium osmotic pressure ( i.e. , positive and negative rates ) give the value π at Ah / At = 0 by extrapolation . A shift in the extrapolated line toward lower pressures ...
... pressure ( usually in terms of h ) both above and below the equilibrium osmotic pressure ( i.e. , positive and negative rates ) give the value π at Ah / At = 0 by extrapolation . A shift in the extrapolated line toward lower pressures ...
Page 134
... pressure differences in systems containing very large proteins ( M > 500,000 ) are necessarily small , the entire span of pressures covering perhaps only a few milli- meters of water . Hence , conditions which contribute to small ...
... pressure differences in systems containing very large proteins ( M > 500,000 ) are necessarily small , the entire span of pressures covering perhaps only a few milli- meters of water . Hence , conditions which contribute to small ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein component protein solution radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero