PCR technology: principles and applications for DNA amplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 154
prenatal diagnoses of B-thalassemia have been carried out by direct detection of
the disease-producing mutations after PCR amplification of regions of the B-
globin gene. These methods provide increased accuracy and diagnosis usually ...
prenatal diagnoses of B-thalassemia have been carried out by direct detection of
the disease-producing mutations after PCR amplification of regions of the B-
globin gene. These methods provide increased accuracy and diagnosis usually ...
Page 156
Each sickle B-globin gene has a 381 -bp signature while the BA-globin gene is
demonstrated by the cleaved products of this fragment, 201 -bp and 180-bp
fragments. Both Bs and BA genes always demonstrate 256-bp and 88-bp
fragments.
Each sickle B-globin gene has a 381 -bp signature while the BA-globin gene is
demonstrated by the cleaved products of this fragment, 201 -bp and 180-bp
fragments. Both Bs and BA genes always demonstrate 256-bp and 88-bp
fragments.
Page 158
Dot-blot showing the use of ASO probes on amplified B-globin DNA for prenatal
diagnosis of B- thalassemia. Both parents in the pedigree at the left carry the
nonsense codon 39 mutation as demonstrated by hybridization of their amplified
...
Dot-blot showing the use of ASO probes on amplified B-globin DNA for prenatal
diagnosis of B- thalassemia. Both parents in the pedigree at the left carry the
nonsense codon 39 mutation as demonstrated by hybridization of their amplified
...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing assay automated B-globin base cDNA cell Cetus chromosome CJ CJ CJ CJ Eh clones concentration containing cycles deletion denaturing denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumor
References to this book
Bibliographic information
| Title | PCR technology: principles and applications for DNA amplification Breakthroughs in Molecular Biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | reprint, illustrated |
| Publisher | Oxford University Press, 1994 |
| Original from | the University of Michigan |
| Digitized | 28 Jul 2008 |
| ISBN | 0195098757, 9780195098754 |
| Length | 256 pages |
| Subjects | › › › Gene amplificatin Gene amplification Literary Criticism / European / English, Irish, Scottish, Welsh Polymerase chain reaction Science / Chemistry / General Science / Life Sciences / Biology / Molecular Biology Science / Life Sciences / Genetics & Genomics |
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