PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 172
... HPRT 1.6 Kb mRNA HPRT gene 28 27 26 25 24 23 22 21 13 12 11 11 21 22 ISSI T X Chromosome ( 150 Mb ) DMD p84 XJ1.1 PERT 87 JBir p20 J66 5 ' 1 0.5 1.0 1.5 3 ' DMD gene 2.0 ( Mb ) 14 Kb mRNA Figure 1. Organization of the genes defective in ...
... HPRT 1.6 Kb mRNA HPRT gene 28 27 26 25 24 23 22 21 13 12 11 11 21 22 ISSI T X Chromosome ( 150 Mb ) DMD p84 XJ1.1 PERT 87 JBir p20 J66 5 ' 1 0.5 1.0 1.5 3 ' DMD gene 2.0 ( Mb ) 14 Kb mRNA Figure 1. Organization of the genes defective in ...
Page 174
... HPRT peptide coding sequence and the presence of sufficient HPRT mRNA in most lymphoblastoid cell lines derived from LN patients to allow the PCR amplification of HPRT complementary DNA ( cDNA ) . The overall scheme is illustrated in ...
... HPRT peptide coding sequence and the presence of sufficient HPRT mRNA in most lymphoblastoid cell lines derived from LN patients to allow the PCR amplification of HPRT complementary DNA ( cDNA ) . The overall scheme is illustrated in ...
Page 177
... HPRT cDNA , 20 have performed well when the reaction annealing temperatures and extension times have been optimized . We routinely use 23-25 base oligonucleotides for the cDNA amplifications , although 16- and 18 - mers have functioned ...
... HPRT cDNA , 20 have performed well when the reaction annealing temperatures and extension times have been optimized . We routinely use 23-25 base oligonucleotides for the cDNA amplifications , although 16- and 18 - mers have functioned ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin gene base cDNA cells Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors