PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 166
... aliquot of supernatant and dilute 1:10 with distilled H2O and then use 4 λ of diluted sample in 100 1 PCR reaction . CVS and cultured cells : Wash villi with TE buffer x 2 . To washed villi add 20-30 λ ( depending on amount of villi ) ...
... aliquot of supernatant and dilute 1:10 with distilled H2O and then use 4 λ of diluted sample in 100 1 PCR reaction . CVS and cultured cells : Wash villi with TE buffer x 2 . To washed villi add 20-30 λ ( depending on amount of villi ) ...
Page 217
... aliquot of cellular material collected from vaginal swab # 3 was stained with nuclear fast red and picroindigo carmine . Both intact spermatozoa and epithelial cells are observed . B. After digestion in the absence of DTT , the ...
... aliquot of cellular material collected from vaginal swab # 3 was stained with nuclear fast red and picroindigo carmine . Both intact spermatozoa and epithelial cells are observed . B. After digestion in the absence of DTT , the ...
Page 221
... aliquot of the pellet for microscopic examination . Suspend pellet in digestion buffer and treat with proteinase K as described above . Digest for no more than 2 hr . Spin in microcentrifuge 1 min to pellet sperm heads . Remove ...
... aliquot of the pellet for microscopic examination . Suspend pellet in digestion buffer and treat with proteinase K as described above . Digest for no more than 2 hr . Spin in microcentrifuge 1 min to pellet sperm heads . Remove ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin gene base cDNA cells Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors