PCR technology: principles and applications for DNA amplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 79
Ideally, the nucleotide sequences of the primers should be at least 50% G + C
and should not contain indirectly repeated sequences. 2. Design one of the two
PCR primers with an additional 40 nt of 100% G + C at its 5' end to serve as the ...
Ideally, the nucleotide sequences of the primers should be at least 50% G + C
and should not contain indirectly repeated sequences. 2. Design one of the two
PCR primers with an additional 40 nt of 100% G + C at its 5' end to serve as the ...
Page 129
-A [B]-~ --[a] -b~ The frequency of these "false recombinants" will be a function of
1) the frequency of samples containing double sperm, and 2) the probability of
amplifying an allele to a detectable level. The lowest frequency of recombination
...
-A [B]-~ --[a] -b~ The frequency of these "false recombinants" will be a function of
1) the frequency of samples containing double sperm, and 2) the probability of
amplifying an allele to a detectable level. The lowest frequency of recombination
...
Page 221
HCl, pH 8.0, 10 mM EDTA, 50 mM NaCl, 2% SDS • Water-saturated n-butanol
Digestion Procedures A. Blood Digestion • Suspend buffy coat, 0.1 ml whole
blood, or blood stain in 0.5 ml digestion buffer containing 15 |il proteinase K
solution.
HCl, pH 8.0, 10 mM EDTA, 50 mM NaCl, 2% SDS • Water-saturated n-butanol
Digestion Procedures A. Blood Digestion • Suspend buffy coat, 0.1 ml whole
blood, or blood stain in 0.5 ml digestion buffer containing 15 |il proteinase K
solution.
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Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing assay automated B-globin base cDNA cell Cetus chromosome CJ CJ CJ CJ Eh clones concentration containing cycles deletion denaturing denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumor
References to this book
Bibliographic information
| Title | PCR technology: principles and applications for DNA amplification Breakthroughs in Molecular Biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | reprint, illustrated |
| Publisher | Oxford University Press, 1994 |
| Original from | the University of Michigan |
| Digitized | 28 Jul 2008 |
| ISBN | 0195098757, 9780195098754 |
| Length | 256 pages |
| Subjects | › › › Gene amplificatin Gene amplification Literary Criticism / European / English, Irish, Scottish, Welsh Polymerase chain reaction Science / Chemistry / General Science / Life Sciences / Biology / Molecular Biology Science / Life Sciences / Genetics & Genomics |
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