PCR technology: principles and applications for DNA amplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 155
Ethidium bromide stain of Cvn\ digested amplified DNA product from individuals
with sickle cell trait (AS), normal B-globin genes (AA), and sickle cell anemia (SS)
. Staining is carried out after electrophoresis in a 3% NuSieve agarose, 1% ...
Ethidium bromide stain of Cvn\ digested amplified DNA product from individuals
with sickle cell trait (AS), normal B-globin genes (AA), and sickle cell anemia (SS)
. Staining is carried out after electrophoresis in a 3% NuSieve agarose, 1% ...
Page 216
In the first step, epithelial cells were preferentially lysed in a digestion buffer
containing detergent and protease.33 The sperm heads, which are resistant to
digestion in the absence of a reducing agent, are collected by centrifugation, ...
In the first step, epithelial cells were preferentially lysed in a digestion buffer
containing detergent and protease.33 The sperm heads, which are resistant to
digestion in the absence of a reducing agent, are collected by centrifugation, ...
Page 221
Protocols 221 • Digestion Buffer: 10 mM Tris.HCl, pH 8.0, 10 mM EDTA, 50 mM
NaCl, 2% SDS • Water-saturated n-butanol Digestion Procedures A. Blood
Digestion • Suspend buffy coat, 0.1 ml whole blood, or blood stain in 0.5 ml
digestion ...
Protocols 221 • Digestion Buffer: 10 mM Tris.HCl, pH 8.0, 10 mM EDTA, 50 mM
NaCl, 2% SDS • Water-saturated n-butanol Digestion Procedures A. Blood
Digestion • Suspend buffy coat, 0.1 ml whole blood, or blood stain in 0.5 ml
digestion ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing assay automated B-globin base cDNA cell Cetus chromosome CJ CJ CJ CJ Eh clones concentration containing cycles deletion denaturing denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumor
References to this book
Bibliographic information
| Title | PCR technology: principles and applications for DNA amplification Breakthroughs in Molecular Biology |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | reprint, illustrated |
| Publisher | Oxford University Press, 1994 |
| Original from | the University of Michigan |
| Digitized | 28 Jul 2008 |
| ISBN | 0195098757, 9780195098754 |
| Length | 256 pages |
| Subjects | › › › Gene amplificatin Gene amplification Literary Criticism / European / English, Irish, Scottish, Welsh Polymerase chain reaction Science / Chemistry / General Science / Life Sciences / Biology / Molecular Biology Science / Life Sciences / Genetics & Genomics |
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