PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 54
However , strong inhibition has been observed of more complex regions , and
efficient PCR of these can only be achieved after the ... Taq polymerase will
incorporate c7dGTP efficiently , but inosine should be avoided as a base analog .
However , strong inhibition has been observed of more complex regions , and
efficient PCR of these can only be achieved after the ... Taq polymerase will
incorporate c7dGTP efficiently , but inosine should be avoided as a base analog .
Page 57
Acknowledgments 57 can ease amplific efficient m using pipleaving oories that
using either conventional radiolabeled primers , or fluorescent terminators or
sequencing primers . However , these instruments are only automating the " back
...
Acknowledgments 57 can ease amplific efficient m using pipleaving oories that
using either conventional radiolabeled primers , or fluorescent terminators or
sequencing primers . However , these instruments are only automating the " back
...
Page 238
The role that mismatches between target and primer play in efficient primer
extension is beginning to undergo more ... The efficiency of extension of a primer
: target duplex mismatched at the 3 ' terminal base is expected to depend to a
great ...
The role that mismatches between target and primer play in efficient primer
extension is beginning to undergo more ... The efficiency of extension of a primer
: target duplex mismatched at the 3 ' terminal base is expected to depend to a
great ...
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Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
14 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids activity addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cell changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example exon extension Figure gene genetic genomic human hybridization identified increase individual initial isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield
References to this book
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification Breakthroughs in Molecular Biology Series |
| Author | Henry A. Erlich |
| Editor | Henry A. Erlich |
| Edition | illustrated, reprint |
| Publisher | Oxford University Press, 1992 |
| Original from | the University of Michigan |
| Digitized | 28 Jul 2008 |
| ISBN | 0195098757, 9780195098754 |
| Length | 246 pages |
| Subjects | › › Literary Criticism / European / English, Irish, Scottish, Welsh |
| Export Citation | BiBTeX EndNote RefMan |