PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 55
... ( lane e ) and ( c ) to both dsDNA and ssDNA ( lane f ) . Thus , the ssDNA produced in reaction ( b ) is exclusively of the desired strand and can be used as hybridization probe . To obtain labeled DNA with high specific activity , the ...
... ( lane e ) and ( c ) to both dsDNA and ssDNA ( lane f ) . Thus , the ssDNA produced in reaction ( b ) is exclusively of the desired strand and can be used as hybridization probe . To obtain labeled DNA with high specific activity , the ...
Page 75
... ( lane 1 ) and with ( lane 5 ) a 40 - bp GC - clamp . Three mutant promoter fragments without ( lanes 2-4 ) and with ( lanes 6-8 ) the same 40 - bp GC - clamp . base mutations in a 140 - bp DNA fragment encompassing the mouse beta - major ...
... ( lane 1 ) and with ( lane 5 ) a 40 - bp GC - clamp . Three mutant promoter fragments without ( lanes 2-4 ) and with ( lanes 6-8 ) the same 40 - bp GC - clamp . base mutations in a 140 - bp DNA fragment encompassing the mouse beta - major ...
Page 76
... Lane 1 shows the position of the wild - type homoduplex fragment , which is also present in the remaining four lanes ... lane , the black dot marks the heteroduplex species . The remaining band in each lane is the mutant homoduplex ...
... Lane 1 shows the position of the wild - type homoduplex fragment , which is also present in the remaining four lanes ... lane , the black dot marks the heteroduplex species . The remaining band in each lane is the mutant homoduplex ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin gene base cDNA cells Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors