PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 86
... obtained from the denaturing gradient gel ; no sequence information need be obtained . Similarly , if the system is being used to screen large numbers of individuals for a known mutation or set of mutations , enough data can be obtained ...
... obtained from the denaturing gradient gel ; no sequence information need be obtained . Similarly , if the system is being used to screen large numbers of individuals for a known mutation or set of mutations , enough data can be obtained ...
Page 90
... obtained from Pharmacia . 5. PCR Primers : The primers are usually 18-22 bases in length and dissolved in TE at 10-100 pm / ul . 6. Reverse transcriptase : Mo - MuLV obtained from Bethesda Research Labs at 200 units / μl . Other sources ...
... obtained from Pharmacia . 5. PCR Primers : The primers are usually 18-22 bases in length and dissolved in TE at 10-100 pm / ul . 6. Reverse transcriptase : Mo - MuLV obtained from Bethesda Research Labs at 200 units / μl . Other sources ...
Page 108
... obtained and its identity confirmed by direct DNA sequencing . They suggest that inverse PCR will be useful for walking into the 5 ' or 3 ' flanking regions of transcribed genes , taking advantage of sequence information obtained from ...
... obtained and its identity confirmed by direct DNA sequencing . They suggest that inverse PCR will be useful for walking into the 5 ' or 3 ' flanking regions of transcribed genes , taking advantage of sequence information obtained from ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin gene base cDNA cells Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors