PCR Technology: Principles and Applications for DNA Amplification

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Oxford University Press, 1992 - Literary Criticism - 246 pages
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Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.

From inside the book

 

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Page 157
The same dots are then hybridized with a normal Bglobin sequence to determine
that sufficient DNA was dotted to produce a positive signal . If the assay is
negative with a mutant probe and positive with the normal probe , we know that
the ...
Page 218
3 2 1 4 SAMPLE DO alpha TYPE POSITIVE CONTROL 1 , 1 POSITIVE
CONTROL 2 , 2 POSITIVE CONTROL POSITIVE CONTROL VICTIM ( BLOOD
HUSBAND ( BLOOD ) SUSPECT ( BLOOD ) DEBRIS , E . CELL 1 , 3 , 4 DEBRIS ,
SPERM ...
Page 238
The implications of false positives in the context of HIV - 1 diagnostic assays
prompted us to develop a series of ... Specifically , there should be a small
number of positive controls with a low target molecule concentration , a large
number of ...

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Contents

PART ONE BASIC METHODOLOGY
1
The Design and Optimization of the PCR
7
Taq DNA Polymerase
17
Copyright

14 other sections not shown

Other editions - View all

PCR Technology: Principles and Applications for DNA Amplification
Henry Erlich
Limited preview - 2015
PCR Technology: Principles and Applications for DNA Amplification
Henry A. Erlich
Snippet view - 1989
PCR Technology: Principles and Applications for DNA Amplification
Henry Erlich
No preview available - 1989

Common terms and phrases

Acad Acids activity addition alleles allow Alu repeats amount amplification analysis annealing applications approach assay base blood buffer carried cDNA cell changes Chapter clones concentration containing cycles deletion denaturation described detection determined diagnosis digestion direct discussed disease DNA fragments DNA polymerase DNA sequence dNTP domain effect efficient electrophoresis enzyme Erlich example exon extension Figure gene genetic genomic human hybridization identified increase individual initial isolation known lane limited loci locus markers melting method molecular Mullis mutations Nature observed obtained oligonucleotide PCR product performed polymorphic positive possible preparation present primers probes problem Proc procedure Protocol reaction recombination region restriction Saiki samples Science separate shown single specific sperm step strand studies Taq polymerase techniques temperature template tube usually yield

References to this book

About the author (1992)

Henry A. Erlich, Senior Scientist and Director, Department of Human Genetics, Cetus Corporation.

Bibliographic information

QR code for PCR Technology
TitlePCR Technology: Principles and Applications for DNA Amplification
Breakthroughs in Molecular Biology Series
AuthorHenry A. Erlich
EditorHenry A. Erlich
Editionillustrated, reprint
PublisherOxford University Press, 1992
Original fromthe University of Michigan
Digitized28 Jul 2008
ISBN0195098757, 9780195098754
Length246 pages
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Literary Criticism / European / English, Irish, Scottish, Welsh
  
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