PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
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Page 53
... reverse transcriptase . This procedure has more limited applicability since it involves additional enzymatic steps following the amplification reaction , and is restricted to using reverse transcriptase as sequencing enzyme . SEQUENCING ...
... reverse transcriptase . This procedure has more limited applicability since it involves additional enzymatic steps following the amplification reaction , and is restricted to using reverse transcriptase as sequencing enzyme . SEQUENCING ...
Page 91
... reverse transcriptase . Incubate 10 min at room temp , then 30-60 min at 42 ° C . To stop reaction , heat tube in 95 ° C water bath for 5-10 min , then quick chill on ice . Note that other manufacturers of reverse transcriptase ...
... reverse transcriptase . Incubate 10 min at room temp , then 30-60 min at 42 ° C . To stop reaction , heat tube in 95 ° C water bath for 5-10 min , then quick chill on ice . Note that other manufacturers of reverse transcriptase ...
Page 92
... reverse transcriptase does not seem crucial to the success of the protocol . MuLV reverse transcriptase from BRL and Boehringer- Mannheim work well , and the AMV enzyme from Boehringer - Mannheim also gives good results . Although we ...
... reverse transcriptase does not seem crucial to the success of the protocol . MuLV reverse transcriptase from BRL and Boehringer- Mannheim work well , and the AMV enzyme from Boehringer - Mannheim also gives good results . Although we ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin gene base cDNA cells Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors