PCR Technology: Principles and Applications for DNA AmplificationPolymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for. |
From inside the book
Results 1-3 of 28
Page 11
... usually optimal ( with 200 μM each dNTP ) , but in some circumstances , different amounts of Mg2 + may prove to be necessary ( Figure 2 ) . Generally , excess Mg2 + will result in the accumulation of non - specific amplification ...
... usually optimal ( with 200 μM each dNTP ) , but in some circumstances , different amounts of Mg2 + may prove to be necessary ( Figure 2 ) . Generally , excess Mg2 + will result in the accumulation of non - specific amplification ...
Page 81
... usually between 55-65 ° C , and then extend at the highest temperature possible , which is usually between 60-72 ° C . These temperatures are determined empirically for each pair of oligonucleotides . 2. The use of longer ( 25-30 nt vs ...
... usually between 55-65 ° C , and then extend at the highest temperature possible , which is usually between 60-72 ° C . These temperatures are determined empirically for each pair of oligonucleotides . 2. The use of longer ( 25-30 nt vs ...
Page 93
... usually the best overall yields of amplified target sequence1011 ( E.S.K. , unpublished results ) . After the first strand synthesis , removal of the RNA template by alkali or RNase treatment is not necessary . The 95 ° C heat treatment ...
... usually the best overall yields of amplified target sequence1011 ( E.S.K. , unpublished results ) . After the first strand synthesis , removal of the RNA template by alkali or RNase treatment is not necessary . The 95 ° C heat treatment ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
Taq DNA Polymerase | 17 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin gene base cDNA cells Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method mismatch molecular molecules mRNA Mullis Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR analysis PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf single sperm Sninsky Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors