Culturing Nerve Cells

Front Cover
Gary Banker, Kimberly Goslin, Medical Director Kimberly Goslin
MIT Press, 1998 - Medical - 666 pages

A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.

Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F.A. Finley, Gerald D. Fishbach, Beat H. Gähwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I. Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones, Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Pröschel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson, Mu-ming Poo, Richard T. Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao

From inside the book

What people are saying - Write a review

We haven't found any reviews in the usual places.


Getting Started
Types of Nerve Cell Cultures Their Advantages and Limitations
Primary Dissociated Cell Cultures
Transfecting Cultured Neurons
Characterizing and Studying Neuronal Cultures
Choosing the Right System
Neuronlike Cells Derived in Culture from P19 Embryonal Carcinoma
Culturing the Large Neurons of Aplysia
Mass Cultures and Microislands of Neurons from Postnatal Rat Brain
Rat Hippocampal Neurons in LowDensity Culture
Rat Striatal Neurons in LowDensity SerumFree Culture
Cell Culture of Cholinergic and Cholinoceptive Neurons from the Medial
Purification and Coculture of Identified Cell Populations
Organotypic Slice Cultures of Neural Tissue
Culture of Astrocytes Oligodendrocytes and 02A Progenitor Cells
Tissue Culture Methods for the Study of Myelination

Culturing Spinal Neurons and Muscle Cells from Xenopus Embryos
Cultures from Chick Peripheral Ganglia
Culturing Mammalian Sympathoadrenal Derivatives

Other editions - View all

Common terms and phrases

Popular passages

Page 612 - Greene, LA, and Tischler, AS (1976). Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Page 612 - A new technique for the assay of infectivity of human adenovirus 5 DNA.
Page 601 - Chomczynski, P. and Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Page 617 - Jessell TM, Siegel RE, and Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. Proc. Natl. Acad. Sci.
Page 636 - A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303...
Page 614 - Hatten, ME (1985) Neuronal regulation of astroglial morphology and proliferation in vitro. J. Cell Biol.
Page 610 - TC 1994. Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse.
Page 608 - Friedrich, G., and Soriano, P. (1991). Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5, 1513-1523.
Page 626 - Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties, J.
Page 616 - Honig, MG, and Hume, RI (1986). Fluorescent carbocyanine dyes allow living neurons of identified origin to be studied in long-term cultures. J. Cell. Biol. 103, 171-187.

Bibliographic information