Polymerase Chain ReactionHenry A. Erlich, Richard A. Gibbs, Richard Gibbs, Haig H. Kazazian (Jr.), Haig H. Kazazian |
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Results 1-3 of 12
Page 53
... blot analysis in this diagnostic work will not be eliminated , but we expect that the use of Southern blots in 1989 will decline to about 53.
... blot analysis in this diagnostic work will not be eliminated , but we expect that the use of Southern blots in 1989 will decline to about 53.
Page 65
... Southern blot Northern blot mRNA ( 93 % ) mRNA ( 7 % ) 1 RNase A localize PCR appropriate exon PCR mRNA PCR promoter and splice junctions Sequence Confirm restriction digest alteration allele - specific oligonucleotide Screen other ...
... Southern blot Northern blot mRNA ( 93 % ) mRNA ( 7 % ) 1 RNase A localize PCR appropriate exon PCR mRNA PCR promoter and splice junctions Sequence Confirm restriction digest alteration allele - specific oligonucleotide Screen other ...
Page 115
... Southern blot analysis . The remaining mutants ( 34/62 ) from experiment 2 either showed no detectable change on a Southern blot or generated a DNA fragment of appropriate size for gpt following PCR . All 34 mutants were PCR - amplified ...
... Southern blot analysis . The remaining mutants ( 34/62 ) from experiment 2 either showed no detectable change on a Southern blot or generated a DNA fragment of appropriate size for gpt following PCR . All 34 mutants were PCR - amplified ...
Contents
Thermus aquaticus DNA Polymerase | 11 |
Optimization of the Polymerase Chain Reaction | 25 |
Diagnosis of Monogenic Disease Based | 47 |
Copyright | |
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Common terms and phrases
Acad alleles amplified DNA assay B-thalassemia base cDNA Cetus chain reaction PCR chromosome clones codon containing cosmid cycles D.H. Gelfand deletions denaturing gradient gel detection diagnosis direct sequencing disease DNA fragments DNA sequence DNA synthesis dNTP Enzymatic amplification enzyme error exon Faloona Fidelity of DNA G.T. Horn gel electrophoresis genetic genomic DNA H.A. Erlich haplotype HPRT HTLV-I hybridization identification infected K.B. Mullis Kunkel Kwok lane Lerman ligation locus method mismatch molecular molecules mRNA Natl normal Nucleic Acids Res nucleotide oligonucleotide probes patients PCR amplification PCR primers PCR product Poiesz polymerase chain reaction polymorphism Proc protein quence recombinant region retroviral retroviruses RNase-A Saiki samples Scharf Science 239 screening sequence analysis Sfil sickle cell anemia single single-base Sninsky Southern blot specific ẞ-globin strand subcloning Taq DNA polymerase Taq polymerase target technique temperature Thermus aquaticus tion vector viral virus vitro