Polymerase Chain ReactionHenry A. Erlich, Richard A. Gibbs, Richard Gibbs, Haig H. Kazazian (Jr.), Haig H. Kazazian |
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Page 53
... disease . The possibilities for use of PCR in diagnosis of monogenic diseases are exceedingly great . PCR - based diagnosis by analy- sis of linked DNA polymorphisms is being used in diagnostic testing for hemophilia , cystic fibrosis ...
... disease . The possibilities for use of PCR in diagnosis of monogenic diseases are exceedingly great . PCR - based diagnosis by analy- sis of linked DNA polymorphisms is being used in diagnostic testing for hemophilia , cystic fibrosis ...
Page 129
... Disease Susceptibility The third area where PCR - based analysis of class II poly- morphisms has proved very valuable is the study of genetic susceptibility to HLA - associated diseases . A variety of diseases have been associated with ...
... Disease Susceptibility The third area where PCR - based analysis of class II poly- morphisms has proved very valuable is the study of genetic susceptibility to HLA - associated diseases . A variety of diseases have been associated with ...
Page 133
... diseases , and yet precise localization of disease- associated MHC genes , until recently , has remained elusive . This is mainly because of the observed linkage disequilibrium between MHC genes , which has made linkage analysis within ...
... diseases , and yet precise localization of disease- associated MHC genes , until recently , has remained elusive . This is mainly because of the observed linkage disequilibrium between MHC genes , which has made linkage analysis within ...
Contents
Thermus aquaticus DNA Polymerase | 11 |
Optimization of the Polymerase Chain Reaction | 25 |
Diagnosis of Monogenic Disease Based | 47 |
Copyright | |
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Common terms and phrases
Acad alleles amplified DNA assay B-thalassemia base cDNA Cetus chain reaction PCR chromosome clones codon containing cosmid cycles D.H. Gelfand deletions denaturing gradient gel detection diagnosis direct sequencing disease DNA fragments DNA sequence DNA synthesis dNTP Enzymatic amplification enzyme error exon Faloona Fidelity of DNA G.T. Horn gel electrophoresis genetic genomic DNA H.A. Erlich haplotype HPRT HTLV-I hybridization identification infected K.B. Mullis Kunkel Kwok lane Lerman ligation locus method mismatch molecular molecules mRNA Natl normal Nucleic Acids Res nucleotide oligonucleotide probes patients PCR amplification PCR primers PCR product Poiesz polymerase chain reaction polymorphism Proc protein quence recombinant region retroviral retroviruses RNase-A Saiki samples Scharf Science 239 screening sequence analysis Sfil sickle cell anemia single single-base Sninsky Southern blot specific ẞ-globin strand subcloning Taq DNA polymerase Taq polymerase target technique temperature Thermus aquaticus tion vector viral virus vitro