PCR Technology: Principles and Applications for DNA Amplification

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Springer, Dec 31, 2015 - Science - 246 pages
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This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
 

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Contents

PART ONE BASIC METHODOLOGY
1
Taq DNA Polymerase
17
PCR Automation
23
Simple and Rapid Preparation
31
PART TWO RESEARCH APPLICATIONS
39
Using PCR to Engineer DNA
61
Mutation Detection by PCR GCClamps
71
Detection of Gene Expression
89
The Use of Repeat Sequence
113
A New Approach to Constructing Genetic
119
Evolutionary Analysis via PCR
137
PART THREE MEDICAL APPLICATIONS
149
Diagnosis of New Mutation Diseases Using
171
Applications of PCR to the Analysis
209
Detection of ras Oncogenes Using PCR
225
Application of PCR to the Detection
235

Inverse Polymerase Chain Reaction
105

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