PCR Technology: Principles and Applications for DNA Amplification
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
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The Use of Repeat Sequence
A New Approach to Constructing Genetic
Evolutionary Analysis via PCR
PART THREE MEDICAL APPLICATIONS
Diagnosis of New Mutation Diseases Using
Applications of PCR to the Analysis
Detection of ras Oncogenes Using PCR
Application of PCR to the Detection
Inverse Polymerase Chain Reaction
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Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin base cDNA cell Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP domain dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method MgCl mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf sequence information single sperm Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors