PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Contents
| 1 | |
Taq DNA Polymerase | 17 |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
Detection of Gene Expression | 89 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res aliquot alleles amplification amplification products amplified DNA annealing Arnheim assay automated B-globin base cDNA cell Cetus chromosome clones concentration containing cycles deletion denaturation denaturing gradient gel detection DGGE diagnosis digestion direct sequencing disease DNA fragments DNA sequence dNTP domain dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis genetic genomic genomic DNA heteroduplex Higuchi HPRT human hybridization identified incubation individual inverse PCR lane loci locus markers melting method MgCl mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers oligonucleotide probes PCR amplification PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphic primers Proc procedure protein Protocol ras gene recombination region restriction enzyme reverse transcriptase Saiki Scharf sequence information single sperm Southern blot specific sperm ssDNA strand synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube tumors
Bibliographic information
| Title | PCR Technology: Principles and Applications for DNA Amplification |
| Author | Henry Erlich |
| Edition | illustrated |
| Publisher | Springer, 2015 |
| ISBN | 1349202355, 9781349202355 |
| Length | 246 pages |
| Subjects | › › Science / Biotechnology Science / Life Sciences / Genetics & Genomics |
| Export Citation | BiBTeX EndNote RefMan |