PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page iii
... Arnheim ) who first developed it for the analysis of human genetic variation . To David Gelfand and Susanne Stoffel who isolated and characterized the thermostable DNA polymerase ( Taq polymerase ) whose use in the PCR led to the ...
... Arnheim ) who first developed it for the analysis of human genetic variation . To David Gelfand and Susanne Stoffel who isolated and characterized the thermostable DNA polymerase ( Taq polymerase ) whose use in the PCR led to the ...
Page v
... Arnheim , Ph.D. Professor of Molecular Biology Department of Biological Sciences University of Southern California Edward T. Blake , D. Crim . Forensic Science Associates Johannes L. Bos , Ph.D. Professor Sylvius Laboratoria University ...
... Arnheim , Ph.D. Professor of Molecular Biology Department of Biological Sciences University of Southern California Edward T. Blake , D. Crim . Forensic Science Associates Johannes L. Bos , Ph.D. Professor Sylvius Laboratoria University ...
Page x
... Arnheim 13. Evolutionary Analysis via PCR 137 Thomas D. Kocher and Thomas J. White PART THREE MEDICAL APPLICATIONS 149 14 . Use of PCR in the Diagnosis of Monogenic Disease Haig H. Kazazian , Jr. 153 15. Diagnosis of New Mutation ...
... Arnheim 13. Evolutionary Analysis via PCR 137 Thomas D. Kocher and Thomas J. White PART THREE MEDICAL APPLICATIONS 149 14 . Use of PCR in the Diagnosis of Monogenic Disease Haig H. Kazazian , Jr. 153 15. Diagnosis of New Mutation ...
Page 5
... Arnheim , N. , and Erlich , H.A. ( 1987 ) New Engl . J. Med . 316 : 656 . 6. Saiki , R.K. , Gelfand , D.H. , Stoffel ... Arnheim , N. ( 1988 ) Nature 335 : 414 . 14. Lench , N. , Stanier , P. , and Williamson , R. ( 1987 ) Lancet ii ...
... Arnheim , N. , and Erlich , H.A. ( 1987 ) New Engl . J. Med . 316 : 656 . 6. Saiki , R.K. , Gelfand , D.H. , Stoffel ... Arnheim , N. ( 1988 ) Nature 335 : 414 . 14. Lench , N. , Stanier , P. , and Williamson , R. ( 1987 ) Lancet ii ...
Page 16
... Arnheim , N. ( 1985 ) Science 37 : 170-172 . 2. Mullis , K.B. , Faloona , F.A. , Scharf , S.J. , Saiki , R.K. , Horn , G.T. , and Erlich , H.A. ( 1986 ) Cold Spring Harbor Symp . Quant . Biol . 51 : 263-273 . 3. Faloona , F. , and ...
... Arnheim , N. ( 1985 ) Science 37 : 170-172 . 2. Mullis , K.B. , Faloona , F.A. , Scharf , S.J. , Saiki , R.K. , Horn , G.T. , and Erlich , H.A. ( 1986 ) Cold Spring Harbor Symp . Quant . Biol . 51 : 263-273 . 3. Faloona , F. , and ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube