PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 1
... method , which was invented by Kary Mullis , 12 was originally applied by a group in the Human Genetics Department at Cetus to the amplification of This human B - globin DNA and to the prenatal diagnosis 1 PART ONE BASIC METHODOLOGY.
... method , which was invented by Kary Mullis , 12 was originally applied by a group in the Human Genetics Department at Cetus to the amplification of This human B - globin DNA and to the prenatal diagnosis 1 PART ONE BASIC METHODOLOGY.
Page 2
... B - globin gene / cell ) and genomic DNA with a homozygous deletion of B - globin were amplified with ẞ - globin primers . Using the normal genomic template , this reaction generated a unique ß- globin fragment with no detectable ...
... B - globin gene / cell ) and genomic DNA with a homozygous deletion of B - globin were amplified with ẞ - globin primers . Using the normal genomic template , this reaction generated a unique ß- globin fragment with no detectable ...
Page 8
... to that of the fragment being amplified . Try to avoid primers with stretches of polypurines , polypyrimidines , or other unusual sequences . Figure 1. Amplification of B - globin fragments ranging from 8 Design and Optimization of the PCR.
... to that of the fragment being amplified . Try to avoid primers with stretches of polypurines , polypyrimidines , or other unusual sequences . Figure 1. Amplification of B - globin fragments ranging from 8 Design and Optimization of the PCR.
Page 9
Principles and Applications for DNA Amplification Henry Erlich. Figure 1. Amplification of B - globin fragments ranging from 150 to 2951 bp . Samples of 100 μl containing standard buffer , 200 μM each dNTP , and 250 nM each primer , 100 ...
Principles and Applications for DNA Amplification Henry Erlich. Figure 1. Amplification of B - globin fragments ranging from 150 to 2951 bp . Samples of 100 μl containing standard buffer , 200 μM each dNTP , and 250 nM each primer , 100 ...
Page 12
... B - globin gene were titrated with various concentrations of MgCl2 . Amplifications were performed as described in Figure 1 except that MgCl2 was varied from 0.5 to 10 mM ( as indicated at the top of each lane ) . Although similar in ...
... B - globin gene were titrated with various concentrations of MgCl2 . Amplifications were performed as described in Figure 1 except that MgCl2 was varied from 0.5 to 10 mM ( as indicated at the top of each lane ) . Although similar in ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube