PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page vi
... Higuchi , Ph.D. Scientist Department of Human Genetics Cetus Corporation Ernest Kawasaki , Ph.D. Scientist Department of Human Genetics Cetus Corporation Haig H. Kazazian , Jr. , M.D. Professor of Pediatrics vi Contributors.
... Higuchi , Ph.D. Scientist Department of Human Genetics Cetus Corporation Ernest Kawasaki , Ph.D. Scientist Department of Human Genetics Cetus Corporation Haig H. Kazazian , Jr. , M.D. Professor of Pediatrics vi Contributors.
Page ix
... Higuchi PART TWO RESEARCH APPLICATIONS 39 5 . Direct Sequencing of In Vitro Amplified DNA Ulf Gyllensten 45 6. Using PCR to Engineer DNA 61 Russell Higuchi 7. Mutation Detection by PCR , GC - Clamps , and Denaturing Gradient Gel ...
... Higuchi PART TWO RESEARCH APPLICATIONS 39 5 . Direct Sequencing of In Vitro Amplified DNA Ulf Gyllensten 45 6. Using PCR to Engineer DNA 61 Russell Higuchi 7. Mutation Detection by PCR , GC - Clamps , and Denaturing Gradient Gel ...
Page x
... Higuchi , George F. Sensabaugh , and Henry A. Erlich Detection of ras Oncogenes Using PCR Johannes L. Bos 235 19. Application of PCR to the Detection of Human Infectious Diseases Shirley Kwok and John J. Sninsky 225 PART ONE BASIC ...
... Higuchi , George F. Sensabaugh , and Henry A. Erlich Detection of ras Oncogenes Using PCR Johannes L. Bos 235 19. Application of PCR to the Detection of Human Infectious Diseases Shirley Kwok and John J. Sninsky 225 PART ONE BASIC ...
Page 5
... Higuchi , R.H. , Horn , G.T. , Mullis , K.B. , and Erlich , H.A. ( 1988 ) Science 239 : 487 . 7. Scharf , S.J. , Horn , G.T. , and Erlich , H.A. ( 1986 ) Science 223 : 1076 . 8. Jeffreys , A.J. , Wilson , V. , Neumann , R. , and Keyte ...
... Higuchi , R.H. , Horn , G.T. , Mullis , K.B. , and Erlich , H.A. ( 1988 ) Science 239 : 487 . 7. Scharf , S.J. , Horn , G.T. , and Erlich , H.A. ( 1986 ) Science 223 : 1076 . 8. Jeffreys , A.J. , Wilson , V. , Neumann , R. , and Keyte ...
Page 22
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube