PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page x
... PCR : The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources 113 David L. Nelson and C. Thomas Caskey A New Approach to Constructing Genetic Maps : PCR Analysis of DNA Sequences in Individual Gametes 119 ...
... PCR : The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources 113 David L. Nelson and C. Thomas Caskey A New Approach to Constructing Genetic Maps : PCR Analysis of DNA Sequences in Individual Gametes 119 ...
Page 1
... primers . Because the primer extension products synthesized in one cycle can serve as a template in the next , the number of target DNA copies approximately doubles at every cycle . Thus , 20 cycles of PCR yields about a million - fold ...
... primers . Because the primer extension products synthesized in one cycle can serve as a template in the next , the number of target DNA copies approximately doubles at every cycle . Thus , 20 cycles of PCR yields about a million - fold ...
Page 2
... PCR used the Klenow fragment of E. coli DNA polymerase I to extend the annealed primers . This enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset of each PCR cycle . Consequently ...
... PCR used the Klenow fragment of E. coli DNA polymerase I to extend the annealed primers . This enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset of each PCR cycle . Consequently ...
Page 3
... PCR results in an improved yield of the amplified target fragment by reducing the competition by non - target products for enzyme and primers . In the later cycles , the amount of enzyme is no longer sufficient to extend all the annealed ...
... PCR results in an improved yield of the amplified target fragment by reducing the competition by non - target products for enzyme and primers . In the later cycles , the amount of enzyme is no longer sufficient to extend all the annealed ...
Page 7
... PCR is primarily due to its apparent simplicity and high probability of success . Reduced to its most basic terms , PCR merely involves combining a DNA sample with oligonucleotide primers , deoxynucleotide triphosphates , and the ...
... PCR is primarily due to its apparent simplicity and high probability of success . Reduced to its most basic terms , PCR merely involves combining a DNA sample with oligonucleotide primers , deoxynucleotide triphosphates , and the ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube