PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page vi
... Product Development , PCR Division Cetus Corporation Richard A. Gibbs , Ph.D. Post - doctoral Fellow Institute for Molecular Genetics Baylor College of Medicine Ulf Gyllensten , Ph.D. Department of Medical Genetics Biomedical Center ...
... Product Development , PCR Division Cetus Corporation Richard A. Gibbs , Ph.D. Post - doctoral Fellow Institute for Molecular Genetics Baylor College of Medicine Ulf Gyllensten , Ph.D. Department of Medical Genetics Biomedical Center ...
Page 2
... PCR is typically analyzed by evaluating the production of the target fragment relative to other products by gel electrophoresis . Another factor influencing the homogeneity of the PCR product is the concentration of the target sequence ...
... PCR is typically analyzed by evaluating the production of the target fragment relative to other products by gel electrophoresis . Another factor influencing the homogeneity of the PCR product is the concentration of the target sequence ...
Page 3
... PCR procedure ( see above ) but significantly increased the specificity and ... PCR results in an improved yield of the amplified target fragment by reducing the ... product concentration may also contribute to the plateau effect and are ...
... PCR procedure ( see above ) but significantly increased the specificity and ... PCR results in an improved yield of the amplified target fragment by reducing the ... product concentration may also contribute to the plateau effect and are ...
Page 4
... PCR to synthesize millions of DNA copies , contamination of the sample reaction with either products of a previous reaction ( product carryover ) or with material from an exogenous source is a potential problem - particularly in those ...
... PCR to synthesize millions of DNA copies , contamination of the sample reaction with either products of a previous reaction ( product carryover ) or with material from an exogenous source is a potential problem - particularly in those ...
Page 10
... PCR product and provide a means of introducing restriction sites or regulatory elements ( e.g. , promoters ) at the ends of the amplified target sequence . " If required , shorter primers or degenerate primers can be used as long as the ...
... PCR product and provide a means of introducing restriction sites or regulatory elements ( e.g. , promoters ) at the ends of the amplified target sequence . " If required , shorter primers or degenerate primers can be used as long as the ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube